Evidence map›Paper›PMID 24789596›Full record

ArticleCurrent protocols in microbiology2014

Murine norovirus: propagation, quantification, and genetic manipulation.

Seungmin Hwang, Bader Alhatlani, Armando Arias, Sarah L Caddy, Constantina Christodoulou, Juliana Bragazza Cunha, Ed Emmott, Marta Gonzalez-Hernandez, Abimbola Kolawole, Jia Lu and 6 more

Open access · bronzeAbstract read
In one paragraph

Article in Current protocols in microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.

0numbers the graph read from it
0cells of the map it votes in
64citing papers in PubMed
4.5field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

64 citing papers in PubMed, 97 citations in OpenAlex.

  1. Article
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  7. Impaired K48-polyubiquitination downmodulates mouse norovirus propagation.Frontiers in cellular and infection microbiology · 2025
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4 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors at 3 institutions in 2 countries.

Seungmin HwangDepartment of Pathology, University of Chicago, Chicago, Illinois.
Bader Alhatlani
Armando Arias
Sarah L Caddy
Constantina Christodoulou
Juliana Bragazza Cunha
Ed Emmott
Marta Gonzalez-Hernandez
Abimbola Kolawole
Jia Lu
Christine Rippinger
Frédéric Sorgeloos
Lucy Thorne
Surender Vashist
Ian Goodfellow
Christiane E Wobus
University of Cambridge · GBUniversity of Michigan–Ann Arbor · USUniversity of Chicago · US

Funding

Re-Engineering Translational Research at the University of ChicagoUL1TR000430 · NCATS · UNIVERSITY OF CHICAGO · PI SOLWAY, JULIAN · 2012 to 2016
$20.2M
Mechanisms of Norovirus EntryR01AI080611 · NIAID · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI WOBUS, CHRISTIANE · 2009 to 2012
$1.5M
Targeting host deubiquitinases for broad spectrum anti-infective therapyR33AI102106 · NIAID · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI O'RIORDAN, MARY · 2014 to 2016
$1.4M
Subversion of gastrointestinal host defenses by norovirusR21AI103961 · NIAID · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI WOBUS, CHRISTIANE · 2013 to 2014
$428k
Targeting host deubiquitinases for broad spectrum anti-infective therapyR21AI102106 · NIAID · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI O'RIORDAN, MARY, WOBUS, CHRISTIANE · 2012 to 2013
$423k
Biotechnology and Biological Sciences Research Council BB/I012303/1Biotechnology and Biological Sciences Research Council BB/K002465/1NCATS NIH HHS UL1 TR000430NIAID NIH HHS AI080611NIAID NIH HHS AI102106NIAID NIH HHS AI103961NIAID NIH HHS R01 AI080611NIAID NIH HHS R21 AI102106NIAID NIH HHS R21 AI103961NIAID NIH HHS R33 AI102106Wellcome Trust 097997Wellcome Trust WT097997MA
6 · The paper itself

Abstract

Murine norovirus (MNV) is a positive-sense, plus-stranded RNA virus in the Caliciviridae family. It is the most common pathogen in biomedical research colonies. MNV is also related to the human noroviruses, which cause the majority of nonbacterial gastroenteritis worldwide. Like the human noroviruses, MNV is an enteric virus that replicates in the intestine and is transmitted by the fecal-oral route. MNV replicates in murine macrophages and dendritic cells in cells in culture and in the murine host. This virus is often used to study mechanisms in norovirus biology, because human noroviruses are refractory to growth in cell culture. MNV combines the availability of a cell culture and reverse genetics system with the ability to study infection in the native host. Herein, we describe a panel of techniques that are commonly used to study MNV biology.

Indexed as

Virus CultivationAnimalsCell LineCentrifugation, Density GradientCesiumChloridesEnzyme-Linked Immunosorbent AssayGenome, ViralHumansMiceMutagenesisNorovirusPolymerase Chain ReactionRecombination, GeneticReverse GeneticsVirus ReplicationCesiumcesium chlorideChloridesmurine noroviruspurificationquantificationreverse geneticstransfection

Identifiers

PMID24789596
PMCPMC4074558
OpenAlexW1903433808

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.