Evidence map›Paper›PMID 24789191›Full record

ArticleJournal of clinical microbiology2014

Development and clinical evaluation of sdaA loop-mediated isothermal amplification assay for detection of Mycobacterium tuberculosis with an approach to prevent carryover contamination.

Manoj Nimesh, Deepali Joon, Mandira Varma-Basil, Daman Saluja

Open access · bronzeAbstract readEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of clinical microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed, 2 pooled it
1.5field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 2 syntheses or guidelines pooled it, 21 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
  3. Review
  4. Review
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 3 institutions in 1 country.

Manoj NimeshDr BR Ambedkar Center for Biomedical Research, University of Delhi, Delhi, India Shri Guru Tegh Bahadur Khalsa College, University of Delhi, Delhi, India.
Deepali JoonDr BR Ambedkar Center for Biomedical Research, University of Delhi, Delhi, India.
Mandira Varma-BasilDepartment of Microbiology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi, India.
Daman SalujaDr BR Ambedkar Center for Biomedical Research, University of Delhi, Delhi, India dsalujach59@gmail.com.
University of Delhi · INAmbedkar University Delhi · INGuru Teg Bahadur Hospital · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A rapid and sensitive loop-mediated isothermal amplification assay for the sdaA gene of Mycobacterium tuberculosis was developed using a dUTP-uracil-N-glycosylase (dUTP-UNG) strategy to prevent carryover contamination. Evaluation of the assay using clinical specimens (n = 648) showed high specificity (97.2%) and sensitivity (100%), demonstrating its potential as a diagnostic test for tuberculosis, especially in resource-limited settings.

Indexed as

HumansMolecular Diagnostic TechniquesMycobacterium tuberculosisNucleic Acid Amplification TechniquesSensitivity and SpecificityTemperatureTime FactorsTuberculosis

Identifiers

PMID24789191
PMCPMC4097723
OpenAlexW1997847658

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.