ArticlePloS one2014
Expanded genetic codes in next generation sequencing enable decontamination and mitochondrial enrichment.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- High throughput single cell analysis of mitochondrial heteroplasmy in mitochondrial diseases.Scientific reports · 2020Article
- A simple method for sequencing the whole human mitochondrial genome directly from samples and its application to genetic testing.Scientific reports · 2019Article
- Rare genetic variants in the endocannabinoid system genes CNR1 and DAGLA are associated with neurological phenotypes in humans.PloS one · 2017Article
- Placental telomere length and risk of placental abruption.The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians · 2016Article
- Metagenomic analysis of medicinalF1000Research · 2016Article
- Placental mitochondrial DNA content and placental abruption: a pilot study.BMC research notes · 2015Article
- Stable heteroplasmy at the single-cell level is facilitated by intercellular exchange of mtDNA.Nucleic acids research · 2015Article
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed a PCR method, coined Déjà vu PCR, that utilizes six nucleotides in PCR with two methyl specific restriction enzymes that respectively digest these additional nucleotides. Use of this enzyme-and-nucleotide combination enables what we term a "DNA diode", where DNA can advance in a laboratory in only one direction and cannot feedback into upstream assays. Here we describe aspects of this method that enable consecutive amplification with the introduction of a 5th and 6th base while simultaneously providing methylation dependent mitochondrial DNA enrichment. These additional nucleotides enable a novel DNA decontamination technique that generates ephemeral and easy to decontaminate DNA.
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