ArticleApplied microbiology and biotechnology2014
Transformable facultative thermophile Geobacillus stearothermophilus NUB3621 as a host strain for metabolic engineering.
Article in Applied microbiology and biotechnology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- An Integrated System for Geobacillus Protoplast Formation, Transfection, Recombinant Genomes Rebooting, Efficient Propagation, and Genomic DNA Isolation of the Thermophilic Bacteriophage TP-84.MicrobiologyOpen · 2026Article
- Unlocking the molecular engineering of Geobacillus glycoside hydrolases as a source of industrial biocatalysts.Biotechnology letters · 2026Review
- The first thermophilic phage display system.Materials today. Bio · 2025Article
- Development of specialized devices for microbial experimental evolution.Development, growth & differentiation · 2024Review
- The Diversity of Bacteriophages in Hot Springs.Methods in molecular biology (Clifton, N.J.) · 2024Review
- A novel thermostable TP-84 capsule depolymerase: a method for rapid polyethyleneimine processing of a bacteriophage-expressed proteins.Microbial cell factories · 2023Article
- New Platform for Screening Genetic Libraries at Elevated Temperatures: Biological and Genomic Information and Genetic Tools of Geobacillus thermodenitrificans K1041.Applied and environmental microbiology · 2022Article
- Genomic insights on fighting bacterial wilt by a novel Bacillus amyloliquefaciens strain Cas02.Microbial biotechnology · 2022Article
- A plasmid vector that directs hyperproduction of recombinant proteins in the thermophiles Geobacillus species.Extremophiles : life under extreme conditions · 2020Article
- Genetic Tools and Techniques for Recombinant Expression in Thermophilic Bacillaceae.Microorganisms · 2018Review
- In vivo selection of sfGFP variants with improved and reliable functionality in industrially important thermophilic bacteria.Biotechnology for biofuels · 2018Article
- Insights into the Geobacillus stearothermophilus species based on phylogenomic principles.BMC microbiology · 2017Article
- Article
- Conjugative plasmid transfer from Escherichia coli is a versatile approach for genetic transformation of thermophilic Bacillus and Geobacillus species.Extremophiles : life under extreme conditions · 2016Article
- Isolation of a genetically accessible thermophilic xylan degrading bacterium from compost.Biotechnology for biofuels · 2016Article
- Isolation and screening of thermophilic bacilli from compost for electrotransformation and fermentation: characterization of Bacillus smithii ET 138 as a new biocatalyst.Applied and environmental microbiology · 2015Article
- Some (bacilli) like it hot: genomics of Geobacillus species.Microbial biotechnology · 2015Article
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3 authors.
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Abstract
Metabolic engineers develop inexpensive enantioselective syntheses of high-value compounds, but their designs are sometimes confounded by the misfolding of heterologously expressed proteins. Geobacillus stearothermophilus NUB3621 is a readily transformable facultative thermophile. It could be used to express and properly fold proteins derived from its many mesophilic or thermophilic Bacillaceae relatives or to direct the evolution of thermophilic variants of mesophilic proteins. Moreover, its capacity for high-temperature growth should accelerate chemical transformation rates in accordance with the Arrhenius equation and reduce the risks of microbial contamination. Its tendency to sporulate in response to nutrient depletion lowers the costs of storage and transportation. Here, we present a draft genome sequence of G. stearothermophilus NUB3621 and describe inducible and constitutive expression plasmids that function in this organism. These tools will help us and others to exploit the natural advantages of this system for metabolic engineering applications.
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