ArticleScientific reports2014
Developmental toxicity of diclofenac and elucidation of gene regulation in zebrafish (Danio rerio).
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 89 citations in OpenAlex.
- Stage-Dependent Embryolethality of Diclofenac Sodium: Quantitative Assessment of Dose-Time Interaction and Critical Windows of Susceptibility in the In Ovo Chicken Embryo Model.Veterinary sciences · 2026Article
- Common antimicrobials disrupt early zebrafish development through immune-cardiac signaling.Environmental science and ecotechnology · 2025Article
- Biotransformation of diclofenac by Stenotrophomonas humi strain DIC_5 and toxicological examination of the resulting metabolites.Applied microbiology and biotechnology · 2024Article
- Combination of advanced nano-Fenton process and sonication for destruction of diclofenac and variables optimization using response surface method.Scientific reports · 2022Article
- Non-steroidal anti-inflammatory drugs in the era of the Covid-19 pandemic in the context of the human and the environment.The Science of the total environment · 2022Review
- Bifidobacterium lactis BL-99 modulates intestinal inflammation and functions in zebrafish models.PloS one · 2022Article
- Presence of diclofenac, estradiol, and ethinylestradiol in Manzanares River (Spain) and their toxicity to zebrafish embryo development.Environmental science and pollution research international · 2021Article
- Evaluation of ibuprofen and diclofenac in the main rivers of Colombia and striped catfish Pseudoplatystoma magdaleniatum.Environmental monitoring and assessment · 2021Article
- Zebrafish toxicity assessment of the photocatalysis-biodegradation of diclofenac using composites of TiOEnvironmental science and pollution research international · 2020Article
- Genotoxicity and Embryotoxicity Study of Bicyclol Methyl Ether, Main Impurity in Bicyclol.Chinese journal of integrative medicine · 2019Article
- As a painkiller: a review of pre- and postnatal non-steroidal anti-inflammatory drug exposure effects on the nervous systems.Inflammopharmacology · 2018Review
- The pathogenesis of diclofenac induced immunoallergic hepatitis in a canine model of liver injury.Oncotarget · 2017Article
- Zebrafish: A Model for the Study of Toxicants Affecting Muscle Development and Function.International journal of molecular sciences · 2016Review
- Oxidative degradation of diclofenac by thermally activated persulfate: implication for ISCO.Environmental science and pollution research international · 2016Article
- Effect of formulation variables on preparation of celecoxib loaded polylactide-co-glycolide nanoparticles.PloS one · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Environmental pollution by emerging contaminants, e.g. pharmaceuticals, has become a matter of widespread concern in recent years. We investigated the membrane transport of diclofenac and its toxic effects on gene expression and the development of zebrafish embryos. The association of diclofenac with the embryos conformed to the general partition model at low concentration, the partition coefficient being 0.0033 ml per embryo. At high concentration, the interaction fitted the Freundlich model. Most of the diclofenac remained in the extracellular aqueous solution with less than 5% interacting with the embryo, about half of which was adsorbed on the membranes while the rest entered the cytoplasm. Concentrations of diclofenac over 10.13 μM were lethal to all the embryos, while 3.78 μM diclofenac was teratogenic. The development abnormalities at 4 day post treatment (dpt) include shorter body length, smaller eye, pericardial and body edema, lack of liver, intestine and circulation, muscle degeneration, and abnormal pigmentation. The portion of the diclofenac transferred into the embryo altered the expression of certain genes, e.g. down-regulation of Wnt3a and Gata4 and up-regulation of Wnt8a. The alteration of expression of such genes or the regulation of downstream genes could cause defects in the cardiovascular and nervous systems.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.