ArticleTissue engineering. Part C, Methods2015
Assessment of an efficient xeno-free culture system of human periodontal ligament stem cells.
Article in Tissue engineering. Part C, Methods, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 46 citations in OpenAlex.
- Effects of Oral Cavity Stem Cell Sources and Serum-Free Cell Culture on Hydrogel Encapsulation of Mesenchymal Stem Cells for Bone Regeneration: An In Vitro Investigation.Bioengineering (Basel, Switzerland) · 2024Article
- Isolation and characterization of human periodontal ligament stem cells under the terms of use in clinical application: A pilot study.Dental research journal · 2023Article
- Brain Homogenate of a Rat Model of Alzheimer's Disease Modifies the Secretome of 3D Cultured Periodontal Ligament Stem Cells: A Potential Neuroregenerative Therapy.Iranian journal of pharmaceutical research : IJPR · 2022Article
- Periodontal ligament stem cells as a promising therapeutic target for neural damage.Stem cell research & therapy · 2022Review
- Optimizing large-scale autologous human keratinocyte sheets for major burns-Toward an animal-free production and a more accessible clinical application.Health science reports · 2022Article
- Pluripotency of Dental Pulp Cells and Periodontal Ligament Cells Was Enhanced through Cell-Cell Communication via STAT3/Oct-4/Sox2 Signaling.Stem cells international · 2021Article
- Human Platelet Lysate Maintains Stemness of Umbilical Cord-Derived Mesenchymal Stromal Cells and Promote Lung Repair in Rat Bronchopulmonary Dysplasia.Frontiers in cell and developmental biology · 2021Article
- Type I collagen facilitates safe and reliable expansion of human dental pulp stem cells in xenogeneic serum-free culture.Stem cell research & therapy · 2020Article
- Comparative effect of platelet-rich plasma, platelet-poor plasma, and fetal bovine serum on the proliferative response of periodontal ligament cell subpopulations.Clinical oral investigations · 2019Article
- Effects of lysophosphatidic acid on human periodontal ligament stem cells from teeth extracted from dental patients.Journal of biomedical research · 2019Article
- Article
- Conditioned medium from human gingival mesenchymal stem cells protects motor-neuron-like NSC-34 cells against scratch-injury-induced cell death.International journal of immunopathology and pharmacology · 2017Article
- MyD88/ERK/NFkB pathways and pro-inflammatory cytokines release in periodontal ligament stem cells stimulated by Porphyromonas gingivalis.European journal of histochemistry : EJH · 2017Article
- Role of Cortico-Cancellous Heterologous Bone in Human Periodontal Ligament Stem Cell Xeno-Free Culture Studied by Synchrotron Radiation Phase-Contrast Microtomography.International journal of molecular sciences · 2017Article
- Stemness Maintenance Properties in Human Oral Stem Cells after Long-Term Passage.Stem cells international · 2017Article
- The secretome of periodontal ligament stem cells from MS patients protects against EAE.Scientific reports · 2016Article
- Alternative source of stem cells derived from human periodontal ligament: a new treatment for experimental autoimmune encephalomyelitis.Stem cell research & therapy · 2016Article
- Human Periodontal Stem Cells Release Specialized Proresolving Mediators and Carry Immunomodulatory and Prohealing Properties Regulated by Lipoxins.Stem cells translational medicine · 2016Article
- Cannabidiol Modulates the Immunophenotype and Inhibits the Activation of the Inflammasome in Human Gingival Mesenchymal Stem Cells.Frontiers in physiology · 2016Article
- Periodontal ligament stem cells: current status, concerns, and future prospects.Stem cells international · 2015Review
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The possibility of transplanting adult stem cells into damaged organs has opened new prospects for the treatment of several human pathologies. The purpose of this study was to develop a culture system for the expansion and production of human Periodontal Ligament Stem Cells (hPDLSCs) using a new xeno-free media formulation and ensuring the maintenance of the stem cells features comprising the multiple passage expansion, mesengenic lineage differentiation, cellular phenotype, and genomic stability, essential elements for conforming to translation to cell therapy. Somatic stem cells were isolated from the human periodontium using a minimally invasive periodontal access flap surgery in healthy donors. Expanded hPDLSCs in a xeno-free culture showed the morphological features of stem cells, expressed the markers associated with pluripotency, and a normal karyotype. Under appropriate culture conditions, hPDLSCs presented adipogenic and osteogenic potential; indeed, a very high accumulation of lipid droplets was evident in the cytoplasm of adipogenic-induced cells, and indisputable evidence of osteogenic differentiation, investigated by transmission electron microscopy, and analyzed for gene expression analysis has been shown. Based on these data, the novel xeno-free culture method might provide the basis for Good Manufacturing Procedure culture of autologous stem cells, readily accessible from human periodontium, and can be a resource to facilitate their use in human clinical studies for potential therapeutic regeneration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.