ArticleBiosensors & bioelectronics2014
An ion-exchange nanomembrane sensor for detection of nucleic acids using a surface charge inversion phenomenon.
Article in Biosensors & bioelectronics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 1 of them a synthesis that pooled it.
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Who cites it
30 citing papers in PubMed, 1 synthesis or guideline pooled it, 76 citations in OpenAlex.
- Electronic and electrochemical viral detection for point-of-care use: A systematic review.PloS one · 2021Pooled it
- Label-free, non-optical detection of intact SARS-CoV-2 virions in native human saliva via microscale surface ion conduction.Biosensors & bioelectronics · 2026Article
- Application of extracellular vesicles in the CRISPR-based diagnosis and treatment: possibilities and challenges.Journal of biological engineering · 2026Review
- Surface markers on supermeres outperform extracellular vesicles in colorectal cancer diagnosis.Scientific reports · 2026Article
- Development and validation of a 5K low-density SNP chip for Hainan cattle.BMC genomics · 2024Article
- Extracellular vesicle and lipoprotein diagnostics (ExoLP-Dx) with membrane sensor: A robust microfluidic platform to overcome heterogeneity.Biomicrofluidics · 2024Article
- An anion exchange membrane sensor detects EGFR and its activity state in plasma CD63 extracellular vesicles from patients with glioblastoma.Communications biology · 2024Article
- Quantifying PON1 on HDL with nanoparticle-gated electrokinetic membrane sensor for accurate cardiovascular risk assessment.Nature communications · 2023Article
- An integrated ion-exchange membrane-based microfluidic device for irreversible dissociation and quantification of miRNA from ribonucleoproteins.Lab on a chip · 2023Article
- Human Heart Anoxia and Reperfusion Tissue (HEART) Model for the Rapid Study of Exosome Bound miRNA Expression As Biomarkers for Myocardial Infarction.Small (Weinheim an der Bergstrasse, Germany) · 2022Article
- Application of a novel lytic phage vB_EcoM_SQ17 for the biocontrol of EnterohemorrhagicFrontiers in microbiology · 2022Article
- Semi-Continuous Desalination and Concentration of Small-Volume Samples.International journal of molecular sciences · 2021Article
- Recent Advances in Nano-Bio-Sensing Fabrication Technology for the Detection of Oral Cancer.Molecular biotechnology · 2021Review
- Water Splitting and Transport of Ions in Electromembrane System with Bilayer Ion-Exchange Membrane.Membranes · 2020Article
- Transitions and Instabilities in Imperfect Ion-Selective Membranes.International journal of molecular sciences · 2020Article
- Label-Free Electrochemical Biosensors for the Determination ofSensors (Basel, Switzerland) · 2020Review
- A numerical study of the selectivity of an isolated cylindrical or conical nanopore to a charged macro-ion.Biomicrofluidics · 2019Article
- Dengue virus: a review on advances in detection and trends - from conventional methods to novel biosensors.Mikrochimica acta · 2019Review
- Extracellular vesicle microRNA quantification from plasma using an integrated microfluidic device.Communications biology · 2019Article
- Nanosensors based on polymer vesicles and planar membranes: a short review.Journal of nanobiotechnology · 2018Review
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
We present a novel low-cost biosensor for rapid, sensitive and selective detection of nucleic acids based on an ionic diode feature of an anion exchange nanoporous membrane under DC bias. The ionic diode feature is associated with external surface charge inversion on the positively charged anion exchange nanomembrane upon hybridization of negatively charged nucleic acid molecules to single-stranded oligoprobes functionalized on the membrane surface resulting in the formation of a cation selective monolayer. The resulting bipolar membrane causes a transition from electroconvection-controlled to water-splitting controlled ion conductance, with a large ion current signature that can be used to accurately quantify the hybridized nucleic acids. The platform is capable of distinguishing two base-pair mismatches in a 22-base pairing segment of microRNAs associated with oral cancer, as well as serotype-specific detection of dengue virus. We also show the sensor' capability to selectively capture target nucleic acids from a heterogeneous mixture. The limit of detection is 1 pM for short 27 base target molecules in a 15-min assay. Similar hybridization results are shown for short DNA molecules as well as RNAs from Brucella and Escherichia coli. The versatility and simplicity of this low-cost biosensor should enable point-of-care diagnostics in food, medical and environmental safety markets.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.