Evidence map›Paper›PMID 24786602›Full record

ArticleBritish journal of cancer2014

Discriminating metastasised from non-metastasised seminoma based on transcriptional changes in primary tumours using NGS.

C G Ruf, H-U Schmelz, M Port, W Wagner, C Matthies, B Müller-Myhsok, V Meineke, M Abend

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in British journal of cancer, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.8field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

C G Ruf1] Department of Urology, Federal Armed Forces Hospital, Hamburg 22049, Germany [2] Bundeswehr Institute of Radiobiology, Munich 80937, Germany.
H-U Schmelz1] Bundeswehr Institute of Radiobiology, Munich 80937, Germany [2] Department of Urology, Federal Armed Forces Central Hospital, Koblenz 56072, Germany.
M Port1] Bundeswehr Institute of Radiobiology, Munich 80937, Germany [2] Department of Hematology, Hemostasis, Oncology and Stem Cell Transplantation, MHH, Hannover, Germany.
W WagnerDepartment of Urology, Federal Armed Forces Hospital, Hamburg 22049, Germany.
C MatthiesDepartment of Urology, Federal Armed Forces Hospital, Hamburg 22049, Germany.
B Müller-MyhsokMax-Plank Institute of Psychiaty, Munich 80804, Germany.
V MeinekeBundeswehr Institute of Radiobiology, Munich 80937, Germany.
M AbendBundeswehr Institute of Radiobiology, Munich 80937, Germany.
Institut für Radiobiologie der Bundeswehr

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundWe aimed to better discriminate (occult) metastasised from non-metastasised seminoma based on transcriptional changes of small RNAs in the primary tumour.

methodsTotal RNAs including small RNAs were isolated from five testicular tumours of each, lymphogenic, occult and non-metastasised patients. Next-generation sequencing (SOLID, Life Technologies) was used to examine transcriptional changes. Small RNAs showing ⩾50 reads and a significant ⩾2-fold difference using non-metastasised tumours as the reference group were examined in univariate logistic regression analysis and combinations of two small RNAs were further examined using support vector machines.

resultsOn average, 1.3 × 10(7), 1.4 × 10(7) and 1.7 × 10(7) small RNA reads were detectable in non-metastasised, occult and lymphogenic metastasised seminoma, respectively, of which 30-32% remained after trimming. Between 59 and 68% represented annotated reads and between 8.6 and 11% were annotated small RNA tags. Of them, 137 small RNAs showed>50 reads and a two-fold difference to the reference. In univariate analysis, 32-38 small RNAs significantly discriminated lymphogenic/occult from non-metastasised seminoma, and among these different comparisons, it were the same small RNAs in 51-88%. Many combinations of two of these small RNAs allowed a complete discrimination of metastasised from non-metastasised seminoma irrespective of the metastasis subtype.

conclusionsMetastasised and non-metastasised seminoma can be completely discriminated with a combination of two small RNAs.

Indexed as

TranscriptomeAdultDiagnosis, DifferentialGene Expression ProfilingHigh-Throughput Nucleotide SequencingHumansLymphatic MetastasisMaleMicroRNAsMiddle AgedMolecular Sequence AnnotationRisk FactorsSeminomaSequence Analysis, RNATesticular NeoplasmsYoung AdultMicroRNAs

Identifiers

PMID24786602
PMCPMC4037819
OpenAlexW2085022418

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-SA
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.