Evidence map›Paper›PMID 24786099›Full record

ArticleInternational journal of molecular sciences2014

Antenna-specific glutathione S-transferase in male silkmoth Bombyx mori.

Xiang Tan, Xiao-Ming Hu, Xiao-Wu Zhong, Quan-Mei Chen, Qing-You Xia, Ping Zhao

Open access · goldAbstract read
In one paragraph

Article in International journal of molecular sciences, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
0.9field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 42 citations in OpenAlex.

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  19. Proteomic Analysis of Silkworm Antennae.Journal of chemical ecology · 2015
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Xiang TanState Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China. xiangtan@swu.edu.cn.
Xiao-Ming HuState Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China. xiaoximingpan@126.com.
Xiao-Wu ZhongMedicine Research Center, North Sichuan Medical College, Nanchong 637000, Sichuan, China. zxw_strive@163.com.
Quan-Mei ChenState Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China. chenquan@swu.edu.cn.
Qing-You XiaState Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China. xiaqy@swu.edu.cn.
Ping ZhaoState Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China. zhaop@swu.edu.cn.
Southwest University · CNNorth Sichuan Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Glutathione S-transferases (GSTs) are multifunctional enzymes that are widely distributed in different species. GSTs detoxify exogenous and endogenous substances by conjugation to reduced glutathione. We characterized BmGSTD4, an antenna-specific GST, in male silkmoths. The full-length mRNA of Bmgstd4 was cloned by RACE-PCR and contained an open reading frame of 738 bp encoding a 245 amino acid protein. The antenna specificity of BmGSTD4 was validated at the mRNA and protein levels and BmGSTD4 was shown to localize in the sensillum of male silkmoth antennae. Homology modeling and multi-sequence alignment suggested that BmGSTD4 was a typical GST belonging to the δ class and had a canonical GST fold with a conserved N-terminus, including a glutathione-binding site and a C-terminal domain harboring a hydrophobic substrate-binding site. Restricted expression of BmGSTD4 in silkmoth antennae combined with GST activity suggested that BmGSTD4 was involved in the detoxification of harmful chemicals.

Indexed as

Amino Acid SequenceAnimalsArthropod AntennaeBombyxCloning, MolecularGlutathione TransferaseMaleModels, MolecularMolecular Sequence DataPhylogenyProtein ConformationRecombinant ProteinsSequence AlignmentGlutathione TransferaseRecombinant Proteins

Identifiers

PMID24786099
PMCPMC4057681
OpenAlexW2124708695

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.