Trial reportTransfusion medicine (Oxford, England)2014
The pro-inflammatory potential of microparticles in red blood cell units.
Trial report in Transfusion medicine (Oxford, England), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 39 citations in OpenAlex.
- Red blood cell transfusion results in adhesion of neutrophils in human endotoxemia and in critically ill patients with sepsis.Transfusion · 2020Trial
- Adverse effects of microparticles on transfusion of stored red blood cell concentrates.Hematology, transfusion and cell therapy · 2024Article
- Reviving ancient therapeutics in modern medicine: an immunological and biological narrative review of wet cupping in the management of Hashimoto's thyroiditis.Gland surgery · 2024Review
- Different-sized extracellular vesicles derived from stored red blood cells package diverse cargoes and cause distinct cellular effects.Transfusion · 2023Article
- Increased Plasma Concentrations of Extracellular Vesicles Are Associated with Pro-Inflammatory and Pro-Thrombotic Characteristics of Left and Right Ventricle Mechanical Support Devices.Journal of cardiovascular development and disease · 2023Article
- The potential effect of leukocyte filtration methods on erythrocyte-derived microvesicles: One step forward.European journal of translational myology · 2022Article
- Storage of packed red blood cells impairs an inherent coagulation property of erythrocytes.Frontiers in physiology · 2022Article
- Materials Selection for the Injection into Vaginal Wall for Treatment of Vaginal Atrophy.Aesthetic plastic surgery · 2021Review
- Platelet-Derived Procoagulant Microparticles as Blood-based Biomarker of Breast Cancer.Asian Pacific journal of cancer prevention : APJCP · 2021Article
- Bedside Allogeneic Erythrocyte Washing with a Cell Saver to Remove Cytokines, Chemokines, and Cell-derived Microvesicles.Anesthesiology · 2021Observational
- Exosomes From Packed Red Cells Induce Human Mast Cell Activation and the Production of Multiple Inflammatory Mediators.Frontiers in immunology · 2021Article
- Procoagulant Activity of Red Blood Cell-Derived Microvesicles during Red Cell Storage.Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie · 2019Article
- Traditional and emerging technologies for washing and volume reducing blood products.Journal of blood medicine · 2019Review
- Red blood cell storage lesion: causes and potential clinical consequences.Blood transfusion = Trasfusione del sangue · 2019Review
- Leukoreduction of packed red blood cells attenuates proinflammatory properties of storage-derived microvesicles.The Journal of surgical research · 2018Article
- Extracellular Vesicles as Markers and Mediators in Sepsis.Theranostics · 2018Review
- The Ability of Extracellular Vesicles to Induce a Pro-Inflammatory Host Response.International journal of molecular sciences · 2017Review
- Duration of red blood cell storage and inflammatory marker generation.Blood transfusion = Trasfusione del sangue · 2017Review
- Acid Sphingomyelinase Inhibition in Stored Erythrocytes Reduces Transfusion-Associated Lung Inflammation.Annals of surgery · 2017Article
- Extracellular Vesicles from Red Blood Cell Products Induce a Strong Pro-Inflammatory Host Response, Dependent on Both Numbers and Storage Duration.Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie · 2016Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundMicroparticles (MPs) are submicron size cell fragments that are released from cells.
objectivesWe hypothesise that MPs increase during red blood cell (RBC) storage and are part of the pro-inflammatory activity, which accumulates in the RBC supernatant. METHODS/MATERIALS: RBC units were separated from whole blood of eight healthy donors: 5 U were split, with 50% undergoing leucoreduction (LR) and the remaining left as unmodified controls. The remaining 3 U were leucoreduced. Samples were obtained at days (D) 1 and 42 and cell-free supernatants separated and stored. The supernatants were centrifuged at 17 000 × g (60 min) or 100 000 × g (120 min) into microparticle-rich (MPR) and microparticle-poor (MPP) portions, resuspended in albumin, incubated with antibodies to CD235 (RBCs), CD45 [white blood cells (WBCs)] and CD41a [platelets (Plts)], and analysed by flow cytometry. Isolated neutrophils were incubated with these samples, and priming activity measured.
resultsTotal MPs increased during storage; however, MPs that marked for precursor cell types did not. Significant priming accumulated in the MPP fraction during storage with some activity present in the MPR fraction from D1 and D42 LR-RBCs.
conclusionMost of the pro-inflammatory priming activity from stored RBCs resides in the MPP supernatant, although the MPR fraction from D42 LR-RBCs does contain some priming activity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.