ArticleNature protocols2014
3D mouse embryonic stem cell culture for generating inner ear organoids.
Article in Nature protocols, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 99 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
99 citing papers in PubMed, 151 citations in OpenAlex.
- Engineering Pluripotent Stem Cells-Derived Inner Ear Organoids With Enhanced Maturation and Reproducibility by Micro-Topographical Cues.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- Inner Ear Organoids: Recent Progress and Challenges.Stem cell reviews and reports · 2026Review
- Bioactive hydrogels for the reconstruction of sensorineural hearing loss.Bioactive materials · 2026Review
- Cryopreserved Human Otic Neuronal Spheroids Self-assemble for Functional Connectivity Analysis and Long-term Ototoxicity Evaluation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Generation and characterization of vestibular inner ear organoids from human pluripotent stem cells.Nature protocols · 2026Review
- Advances and prospects of 3D air-liquid interface cultured middle ear epithelial cells and inner ear organoids in otologic diseases.Frontiers in bioengineering and biotechnology · 2026Review
- Inner Ear Organoids: A Hydrogel-Based Platform for Drug Screening and Deafness Modeling.Neuroscience bulletin · 2026Review
- Design and Synthesis of New Quinoxaline Analogs to Regenerate Sensory Auditory Hair Cells.Cells · 2025Article
- Inner ear organoids: Recent progress and potential applications.Fundamental research · 2025Review
- Study Models for Non-Syndromic Hearing Loss.Cells · 2025Review
- Inner Ear Organoid as a Preclinical Model of Hearing Regeneration: Progress and Applications.Stem cell reviews and reports · 2025Review
- The Reconstruction of Peripheral Auditory Circuit: Recent Advances and Future Challenges.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Advances in Microfluidic Cochlea-On-A-Chip.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Organizational principles of integumentary organs: Maximizing variations for effective adaptation.Developmental biology · 2025Review
- Optimized inner ear organoids for efficient hair cell generation and ototoxicity response modeling.Science China. Life sciences · 2025Article
- Review
- Article
- Functional regeneration strategies of hair follicles: advances and challenges.Stem cell research & therapy · 2025Review
- Article
- Modern In Vitro Techniques for Modeling Hearing Loss.Bioengineering (Basel, Switzerland) · 2024Review
39 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
This protocol describes a culture system in which inner-ear sensory tissue is produced from mouse embryonic stem (ES) cells under chemically defined conditions. This model is amenable to basic and translational investigations into inner ear biology and regeneration. In this protocol, mouse ES cells are aggregated in 96-well plates in medium containing extracellular matrix proteins to promote epithelialization. During the first 14 d, a series of precisely timed protein and small-molecule treatments sequentially induce epithelia that represent the mouse embryonic non-neural ectoderm, preplacodal ectoderm and otic vesicle epithelia. Ultimately, these tissues develop into cysts with a pseudostratified epithelium containing inner ear hair cells and supporting cells after 16-20 d. Concurrently, sensory-like neurons generate synapse-like structures with the derived hair cells. We have designated the stem cell-derived epithelia harboring hair cells, supporting cells and sensory-like neurons as inner ear organoids. This method provides a reproducible and scalable means to generate inner ear sensory tissue in vitro.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.