Evidence map›Paper›PMID 24784448›Full record

ArticleGene therapy2014

Overexpression of Ad5 precursor terminal protein accelerates recombinant adenovirus packaging and amplification in HEK-293 packaging cells.

N Wu, H Zhang, F Deng, R Li, W Zhang, X Chen, S Wen, N Wang, J Zhang, L Yin and 12 more

Open access · bronzeAbstract read
PubMed Publisher
In one paragraph

Article in Gene therapy, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.

0numbers the graph read from it
0cells of the map it votes in
62citing papers in PubMed
6.1field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

62 citing papers in PubMed, 89 citations in OpenAlex.

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2 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

22 authors at 4 institutions in 2 countries.

N Wu1] Stem Cell Biology and Therapy Laboratory, Ministry of Education Key Laboratory for Pediatrics, and Chongqing Stem Cell Therapy and Engineering Center, The Children's Hospital of Chongqing Medical University, Chongqing, China [2] Department of Orthopaedic Surgery, Molecular Oncology Laboratory, University of Chicago Medical Center, Chicago, IL, USA [3] Ministry of Education Key Laboratory of Diagnostic Medicine and School of Clinical Diagnostic Medicine, and the Affiliated Hospitals of Chongqing Medical University, Chongqing, China.
H Zhang1] Department of Orthopaedic Surgery, Molecular Oncology Laboratory, University of Chicago Medical Center, Chicago, IL, USA [2] Chongqing Key Laboratory for Oral Diseases and Biomedical Sciences, and the Affiliated Hospital of Stomatology, Chongqing Medical University, Chongqing, China.
F Deng1] Department of Orthopaedic Surgery, Molecular Oncology Laboratory, University of Chicago Medical Center, Chicago, IL, USA [2] Departments of Cell Biology and Oncology of the Affiliated Southwest Hospital, the Third Military Medical University, Chongqing, China.
R Li1] Department of Orthopaedic Surgery, Molecular Oncology Laboratory, University of Chicago Medical Center, Chicago, IL, USA [2] Ministry of Education Key Laboratory of Diagnostic Medicine and School of Clinical Diagnostic Medicine, and the Affiliated Hospitals of Chongqing Medical University, Chongqing, China.
W Zhang1] Department of Orthopaedic Surgery, Molecular Oncology Laboratory, University of Chicago Medical Center, Chicago, IL, USA [2] Department of Laboratory Medicine, the Affiliated Hospital of Bingzhou Medical University, Yantai, China.
X Chen1] Department of Orthopaedic Surgery, Molecular Oncology Laboratory, University of Chicago Medical Center, Chicago, IL, USA [2] Ministry of Education Key Laboratory of Diagnostic Medicine and School of Clinical Diagnostic Medicine, and the Affiliated Hospitals of Chongqing Medical University, Chongqing, China.
S Wen1] Stem Cell Biology and Therapy Laboratory, Ministry of Education Key Laboratory for Pediatrics, and Chongqing Stem Cell Therapy and Engineering Center, The Children's Hospital of Chongqing Medical University, Chongqing, China [2] Department of Orthopaedic Surgery, Molecular Oncology Laboratory, University of Chicago Medical Center, Chicago, IL, USA.
N Wang1] Department of Orthopaedic Surgery, Molecular Oncology Laboratory, University of Chicago Medical Center, Chicago, IL, USA [2] Departments of Cell Biology and Oncology of the Affiliated Southwest Hospital, the Third Military Medical University, Chongqing, China.
J Zhang1] Department of Orthopaedic Surgery, Molecular Oncology Laboratory, University of Chicago Medical Center, Chicago, IL, USA [2] Ministry of Education Key Laboratory of Diagnostic Medicine and School of Clinical Diagnostic Medicine, and the Affiliated Hospitals of Chongqing Medical University, Chongqing, China.
L Yin1] Department of Orthopaedic Surgery, Molecular Oncology Laboratory, University of Chicago Medical Center, Chicago, IL, USA [2] Ministry of Education Key Laboratory of Diagnostic Medicine and School of Clinical Diagnostic Medicine, and the Affiliated Hospitals of Chongqing Medical University, Chongqing, China.
Z Liao1] Department of Orthopaedic Surgery, Molecular Oncology Laboratory, University of Chicago Medical Center, Chicago, IL, USA [2] Department of Orthopaedic Surgery, the Affiliated Xiang-Ya Hospital of Central South University, Changsha, China.
Z Zhang1] Department of Orthopaedic Surgery, Molecular Oncology Laboratory, University of Chicago Medical Center, Chicago, IL, USA [2] Department of Surgery, the Affiliated Zhongnan Hospital of Wuhan University, Wuhan, China.
Q Zhang1] Stem Cell Biology and Therapy Laboratory, Ministry of Education Key Laboratory for Pediatrics, and Chongqing Stem Cell Therapy and Engineering Center, The Children's Hospital of Chongqing Medical University, Chongqing, China [2] Department of Orthopaedic Surgery, Molecular Oncology Laboratory, University of Chicago Medical Center, Chicago, IL, USA.
Z Yan1] Department of Orthopaedic Surgery, Molecular Oncology Laboratory, University of Chicago Medical Center, Chicago, IL, USA [2] Ministry of Education Key Laboratory of Diagnostic Medicine and School of Clinical Diagnostic Medicine, and the Affiliated Hospitals of Chongqing Medical University, Chongqing, China.
W Liu1] Stem Cell Biology and Therapy Laboratory, Ministry of Education Key Laboratory for Pediatrics, and Chongqing Stem Cell Therapy and Engineering Center, The Children's Hospital of Chongqing Medical University, Chongqing, China [2] Department of Orthopaedic Surgery, Molecular Oncology Laboratory, University of Chicago Medical Center, Chicago, IL, USA.
D WuDepartment of Orthopaedic Surgery, Molecular Oncology Laboratory, University of Chicago Medical Center, Chicago, IL, USA.
J Ye1] Department of Orthopaedic Surgery, Molecular Oncology Laboratory, University of Chicago Medical Center, Chicago, IL, USA [2] School of Bioengineering, Chongqing University, Chongqing, China.
Y Deng1] Department of Orthopaedic Surgery, Molecular Oncology Laboratory, University of Chicago Medical Center, Chicago, IL, USA [2] Ministry of Education Key Laboratory of Diagnostic Medicine and School of Clinical Diagnostic Medicine, and the Affiliated Hospitals of Chongqing Medical University, Chongqing, China.
K Yang1] Stem Cell Biology and Therapy Laboratory, Ministry of Education Key Laboratory for Pediatrics, and Chongqing Stem Cell Therapy and Engineering Center, The Children's Hospital of Chongqing Medical University, Chongqing, China [2] Department of Orthopaedic Surgery, Molecular Oncology Laboratory, University of Chicago Medical Center, Chicago, IL, USA.
H H LuuDepartment of Orthopaedic Surgery, Molecular Oncology Laboratory, University of Chicago Medical Center, Chicago, IL, USA.
R C HaydonDepartment of Orthopaedic Surgery, Molecular Oncology Laboratory, University of Chicago Medical Center, Chicago, IL, USA.
T-C He1] Stem Cell Biology and Therapy Laboratory, Ministry of Education Key Laboratory for Pediatrics, and Chongqing Stem Cell Therapy and Engineering Center, The Children's Hospital of Chongqing Medical University, Chongqing, China [2] Department of Orthopaedic Surgery, Molecular Oncology Laboratory, University of Chicago Medical Center, Chicago, IL, USA [3] Ministry of Education Key Laboratory of Diagnostic Medicine and School of Clinical Diagnostic Medicine, and the Affiliated Hospitals of Chongqing Medical University, Chongqing, China.
University of Chicago Medical Center · USMolecular Oncology (United States) · USChildren's Hospital of Chongqing Medical University · CNChongqing Medical University · CN

Funding

Re-Engineering Translational Research at the University of ChicagoUL1TR000430 · NCATS · UNIVERSITY OF CHICAGO · PI SOLWAY, JULIAN · 2012 to 2016
$20.2M
NCATS NIH HHS UL1 TR000430
6 · The paper itself

Abstract

Recombinant adenoviruses are one of the most common vehicles for efficient in vitro and in vivo gene deliveries. Here, we investigate whether exogenous precursor terminal protein (pTP) expression in 293 cells improves the efficiency of adenovirus packaging and amplification. We used a piggyBac transposon-based vector and engineered a stable 293 line that expresses high level of Ad5 pTP, designated as 293pTP. Using the AdBMP6-GLuc that expresses green fluorescent protein (GFP), BMP6 and Gaussia luciferase, we found that the infectivity of AdBMP6-GLuc viral samples packaged in 293pTP cells was titrated up to 19.3 times higher than that packaged in parental 293 cells. AdBMP6-GLuc viral samples packaged in 293pTP cells exhibited significantly higher transduction efficiency in 143B and immortalized mouse embryonic fibroblast (iMEF) cells, as assessed by fluorescence-activated cell sorting analysis of GFP-positive cells, the luciferase activity assay and BMP6-induced osteogenic marker alkaline phosphatase activities in iMEFs. When adenovirus amplification efficiency was analyzed, we found that 293pTP cells infected with AdBMP6-GLuc yielded up to 12.6 times higher titer than that in parental 293 cells, especially at lower multiplicities of infection. These results strongly suggest that exogenous pTP expression may accelerate the packaging and amplification of recombinant adenoviruses. Thus, the engineered 293pTP cells should be a superior packaging line for efficient adenovirus production.

Indexed as

Virus AssemblyAdenoviridaeAdenoviridae InfectionsAnimalsCell Line, TumorEmbryo, MammalianFibroblastsGenetic VectorsHEK293 CellsHumansMesenchymal Stem CellsMicePhosphoproteinsProtein PrecursorsRecombination, GeneticTransduction, GeneticPhosphoproteinsProtein PrecursorspTP protein, adenovirusViral Proteins

Identifiers

PMID24784448
OpenAlexW2042851200

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.