ArticlePLoS genetics2014
Genetic interactions involving five or more genes contribute to a complex trait in yeast.
Article in PLoS genetics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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Who cites it
54 citing papers in PubMed.
- Environment-by-Environment Interactions Differ Across Genetic Backgrounds.Molecular biology and evolution · 2025Article
- Engineered CRISPR-Cas12a for higher-order combinatorial chromatin perturbations.Nature biotechnology · 2025Article
- Systematic data analysis pipeline for quantitative morphological cell phenotyping.Computational and structural biotechnology journal · 2024Review
- Genome-scale analysis of interactions between genetic perturbations and natural variation.Nature communications · 2024Article
- Genome-scale analysis of interactions between genetic perturbations and natural variation.bioRxiv : the preprint server for biology · 2024Article
- Higher-order epistasis shapes natural variation in germ stem cell niche activity.Nature communications · 2023Article
- Regulatory role of Mss11 inFrontiers in cellular and infection microbiology · 2023Article
- Identification and Characterization of a QTL for Growth ofJournal of fungi (Basel, Switzerland) · 2022Article
- The interplay of additivity, dominance, and epistasis on fitness in a diploid yeast cross.Nature communications · 2022Article
- Genetic basis of a spontaneous mutation's expressivity.Genetics · 2022Article
- NeuralLasso: Neural Networks Meet Lasso in Genomic Prediction.Frontiers in plant science · 2022Article
- Random Penetrance of Mutations Among Individuals: A New Type of Genetic Drift in Molecular Evolution.Phenomics (Cham, Switzerland) · 2021Article
- The complex role of genetic background in shaping the effects of spontaneous and induced mutations.Yeast (Chichester, England) · 2021Review
- τ-SGA: synthetic genetic array analysis for systematically screening and quantifying trigenic interactions in yeast.Nature protocols · 2021Article
- Pleiotropy and epistasis within and between signaling pathways defines the genetic architecture of fungal virulence.PLoS genetics · 2021Article
- Genetic buffering and potentiation in metabolism.PLoS computational biology · 2020Article
- Toxo: a library for calculating penetrance tables of high-order epistasis models.BMC bioinformatics · 2020Article
- A Gaussian process-based definition reveals new and bona fide genetic interactions compared to a multiplicative model in the Gram-negative Escherichia coli.Bioinformatics (Oxford, England) · 2020Article
- Highly Combinatorial Genetic Interaction Analysis Reveals a Multi-Drug Transporter Influence Network.Cell systems · 2020Article
- Polygenic analysis of very high acetic acid tolerance in the yeastBiotechnology for biofuels · 2020Article
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2 authors.
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Abstract
Recent research suggests that genetic interactions involving more than two loci may influence a number of complex traits. How these 'higher-order' interactions arise at the genetic and molecular levels remains an open question. To provide insights into this problem, we dissected a colony morphology phenotype that segregates in a yeast cross and results from synthetic higher-order interactions. Using backcrossing and selective sequencing of progeny, we found five loci that collectively produce the trait. We fine-mapped these loci to 22 genes in total and identified a single gene at each locus that caused loss of the phenotype when deleted. Complementation tests or allele replacements provided support for functional variation in these genes, and revealed that pre-existing genetic variants and a spontaneous mutation interact to cause the trait. The causal genes have diverse functions in endocytosis (END3), oxidative stress response (TRR1), RAS-cAMP signalling (IRA2), and transcriptional regulation of multicellular growth (FLO8 and MSS11), and for the most part have not previously been shown to exhibit functional relationships. Further efforts uncovered two additional loci that together can complement the non-causal allele of END3, suggesting that multiple genotypes in the cross can specify the same phenotype. Our work sheds light on the complex genetic and molecular architecture of higher-order interactions, and raises questions about the broader contribution of such interactions to heritable trait variation.
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