ArticleBioMed research international2014
Recombinant keratinocyte growth factor 1 in tobacco potentially promotes wound healing in diabetic rats.
Article in BioMed research international, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 26 citations in OpenAlex.
- Exploring Naturally-Derived Targeted Nano Delivery Therapy for Burn Wound Healing with Special Emphasis on Preclinical Outcomes.Current drug delivery · 2025Review
- A novel 22-bp InDel withinAnimal biotechnology · 2024Article
- Production of a 135-residue long N-truncated human keratinocyte growth factor 1 in Escherichia coli.Microbial cell factories · 2023Article
- Phytochemical-Based Nano-Pharmacotherapeutics for Management of Burn Wound Healing.Gels (Basel, Switzerland) · 2021Review
- Expression optimization, purification andBiotechnology reports (Amsterdam, Netherlands) · 2020Article
- Transient expression of human serum albumin (HSA) in tobacco leaves.Molecular biology reports · 2020Article
- Nanomedicine and advanced technologies for burns: Preventing infection and facilitating wound healing.Advanced drug delivery reviews · 2018Review
- Local delivery of recombinant human FGF7 enhances bone formation in rat mandible defects.Journal of bone and mineral metabolism · 2017Article
- The influence of growth factors on skin wound healing in rats.Brazilian journal of otorhinolaryngologyArticle
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Keratinocyte growth factor 1 (KGF1) is a growth factor that promotes epidermal cell proliferation, migration, differentiation, and wound repair. It is expressed at low levels in a form of inclusion body in E. coli. In order to increase its expression and activity, we produced tobacco plants expressing KGF1 via Agrobacterium-mediated transformation using a potato virus X (PVX)-based vector (pgR107). The vector contained the sequence encoding the KGF1 gene fused with a green florescence protein. The recombinant plasmid was introduced into leaf cells of Nicotiana benthamiana (a wild Australian tobacco) via Agrobacterium-mediated agroinfiltration. As determined by fluorescence and Western blot of leaf extracts, the KGF1 gene was correctly translated into the tobacco plants. The recombinant KGF1 was purified from plant tissues by heparin affinity chromatography, and cell proliferation in NIH/3T3 cells was stimulated by the purified KGF1. The purified KGF1 was also applied to the wounds of type-II diabetic rats. KGF1 had accumulated to levels as high as 530 μ g/g fresh weight in the leaves of agroinfected plants. We show that plant-derived KGF1 can promote the proliferation of NIH/3T3 cells and have significant effects on the type-II diabetic rat. The present findings indicated that KGF1 from tobacco maintains its biological activity, implying prospective industrial production in a plant bioreactor.
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