ArticleNucleic acids research2014
Molecular determinants of the DprA-RecA interaction for nucleation on ssDNA.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 28 citations in OpenAlex.
- The molecular mechanisms of the ShosTA system in mediating anti-phage defense.Nucleic acids research · 2026Article
- A Single DNA Binding Site of DprA Dimer Is Required to Facilitate RecA Filament Nucleation.International journal of molecular sciences · 2025Article
- DprA recruits ComM to facilitate recombination during natural transformation in Gram-negative bacteria.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- The acquisition of clinically relevant amoxicillin resistance in Streptococcus pneumoniae requires ordered horizontal gene transfer of four loci.PLoS pathogens · 2022Article
- Competence shut-off by intracellular pheromone degradation in salivarius streptococci.PLoS genetics · 2022Article
- Structural insight into the length-dependent binding of ssDNA by SP_0782 from Streptococcus pneumoniae, reveals a divergence in the DNA-binding interface of PC4-like proteins.Nucleic acids research · 2020Article
- Natural Transformation inFrontiers in microbiology · 2020Article
- RecA Regulation by RecU and DprA DuringFrontiers in microbiology · 2018Article
- Bacillus subtilis RecA with DprA-SsbA antagonizes RecX function during natural transformation.Nucleic acids research · 2017Article
- DprA from Neisseria meningitidis: properties and role in natural competence for transformation.Microbiology (Reading, England) · 2017Article
- Comparative proteomic analysis of Neisseria meningitidis wildtype and dprA null mutant strains links DNA processing to pilus biogenesis.BMC microbiology · 2017Article
- InterEvDock: a docking server to predict the structure of protein-protein interactions using evolutionary information.Nucleic acids research · 2016Article
- Comparative genomic analysis of six new-found integrative conjugative elements (ICEs) in Vibrio alginolyticus.BMC microbiology · 2016Article
- Insights into the Functional Roles of N-Terminal and C-Terminal Domains of Helicobacter pylori DprA.PloS one · 2015Article
- Roles of Bacillus subtilis DprA and SsbA in RecA-mediated genetic recombination.The Journal of biological chemistry · 2014Article
- The virulence of Streptococcus pneumoniae partially depends on dprA.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]Article
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Natural transformation is a major mechanism of horizontal gene transfer in bacteria that depends on DNA recombination. RecA is central to the homologous recombination pathway, catalyzing DNA strand invasion and homology search. DprA was shown to be a key binding partner of RecA acting as a specific mediator for its loading on the incoming exogenous ssDNA. Although the 3D structures of both RecA and DprA have been solved, the mechanisms underlying their cross-talk remained elusive. By combining molecular docking simulations and experimental validation, we identified a region on RecA, buried at its self-assembly interface and involving three basic residues that contact an acidic triad of DprA previously shown to be crucial for the interaction. At the core of these patches, (DprA)M238 and (RecA)F230 are involved in the interaction. The other DprA binding regions of RecA could involve the N-terminal α-helix and a DNA-binding region. Our data favor a model of DprA acting as a cap of the RecA filament, involving a DprA-RecA interplay at two levels: their own oligomeric states and their respective interaction with DNA. Our model forms the basis for a mechanistic explanation of how DprA can act as a mediator for the loading of RecA on ssDNA.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.