ArticleNucleic acids research2014
A family of synthetic riboswitches adopts a kinetic trapping mechanism.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 32 citations in OpenAlex.
- Single Molecule Force Measurements Reveal Energy Stabilization of Theophylline Aptamer RNA Switch Required for Gene Control.ACS physical chemistry Au · 2026Article
- Structural insights into lab-coevolved RNA-RBP pairs and applications of synthetic riboswitches in cell-free system.Nucleic acids research · 2025Article
- Rational design of eukaryotic riboswitches that up-regulate IRES-mediated translation initiation with high switching efficiency through a kinetic trapping mechanism in vitro.RNA (New York, N.Y.) · 2023Article
- Riboswitches, from cognition to transformation.Synthetic and systems biotechnology · 2023Review
- High-throughput screening of cell-free riboswitches by fluorescence-activated droplet sorting.Nucleic acids research · 2022Article
- Article
- Cell-free riboswitches.RSC chemical biology · 2021Review
- Attempted use of PACE for riboswitch discovery generates three new translational theophylline riboswitch side products.BMC research notes · 2018Article
- Recent advances and future trends of riboswitches: attractive regulatory tools.World journal of microbiology & biotechnology · 2018Review
- Design of Artificial Riboswitches as Biosensors.Sensors (Basel, Switzerland) · 2017Review
- Characterization of Engineered PreQ1 Riboswitches for Inducible Gene Regulation in Mycobacteria.Journal of bacteriology · 2017Article
- Landscape of RNAs in human lumbar disc degeneration.Oncotarget · 2016Article
- Opportunities in the design and application of RNA for gene expression control.Nucleic acids research · 2016Review
- Automated physics-based design of synthetic riboswitches from diverse RNA aptamers.Nucleic acids research · 2016Article
- Quantitative and predictive model of kinetic regulation by E. coli TPP riboswitches.RNA biology · 2016Article
- Design criteria for synthetic riboswitches acting on transcription.RNA biology · 2015Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Riboswitches are sequences of RNA that control gene expression via RNA-ligand interactions, without the need for accessory proteins. Riboswitches consist of an aptamer that recognizes the ligand and an expression platform that couples ligand binding to a change in gene expression. Using in vitro selection, it is possible to screen large (∼ 10(13) members) libraries of RNA sequences to discover new aptamers. However, limitations in bacterial transformation efficiency make screening such large libraries for riboswitch function in intact cells impractical. Here we show that synthetic riboswitches function in an E. coli S30 extract in a manner similar to how they function in intact E. coli cells. We discovered that, although this family of riboswitches regulates the initiation of protein translation, the fate of whether an RNA message is translated is determined during transcription. Thus, ligand binding does not bias a population of rapidly equilibrating RNA structures, but rather, co-transcriptional ligand binding kinetically traps the RNA in a conformation that supports efficient translation. In addition to providing new insights into the mechanisms of action of a family of synthetic riboswitches, our experiments suggest that it may be possible to perform selections for novel synthetic riboswitches in an in vitro system.
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