ArticleChemphyschem : a European journal of chemical physics and physical chemistry2014
Far-red organic fluorophores contain a fluorescent impurity.
Article in Chemphyschem : a European journal of chemical physics and physical chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed.
- Photophysics of Organic Fluorophore Photobluing and Its Applications in Fluorescence and Super-Resolution Microscopy.Accounts of chemical research · 2026Article
- Cyanines Substituted on the Polymethine Chain: Synthesis, Resulting Properties, and Application Use Cases.ChemPlusChem · 2025Review
- Ethyl Cinnamate-Based Tissue Clearing Technique on Vocal Fold Mucosa with Vasculature Model.Journal of voice : official journal of the Voice Foundation · 2025Article
- Super-photostable organic dye for long-term live-cell single-protein imaging.Nature methods · 2025Article
- Multiplexed Nanoscopy via Buffer Exchange.ACS nano · 2024Article
- Thermal Truncation of Heptamethine Cyanine Dyes.Journal of the American Chemical Society · 2024Article
- Photochemical Mechanisms of Fluorophores Employed in Single-Molecule Localization Microscopy.Angewandte Chemie (International ed. in English) · 2023Review
- Measuring the Co-Localization and Dynamics of Mobile Proteins in Live Cells Undergoing Signaling Responses.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Defining the Basis of Cyanine Phototruncation Enables a New Approach to Single-Molecule Localization Microscopy.ACS central science · 2021Article
- Blue-conversion of organic dyes produces artifacts in multicolor fluorescence imaging.Chemical science · 2021Article
- Photoblueing of organic dyes can cause artifacts in super-resolution microscopy.Nature methods · 2021Article
- Synergistic factors control kinase-phosphatase organization in B-cells engaged with supported bilayers.Molecular biology of the cell · 2020Article
- Method to identify and minimize artifacts induced by fluorescent impurities in single-molecule localization microscopy.Journal of biomedical optics · 2018Article
- Optimization of fluorophores for chemical tagging and immunohistochemistry of Drosophila neurons.PloS one · 2018Article
- Super-Resolution Microscopy: Shedding Light on the Cellular Plasma Membrane.Chemical reviews · 2017Review
- Article
- The Continuing Mystery of Lipid Rafts.Journal of molecular biology · 2016Review
- Functional nanoscale coupling of Lyn kinase with IgE-FcεRI is restricted by the actin cytoskeleton in early antigen-stimulated signaling.Molecular biology of the cell · 2016Article
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2 authors.
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Abstract
Far-red organic fluorophores commonly used in traditional and super-resolution localization microscopy are found to contain a fluorescent impurity with green excitation and near-red emission. This near-red fluorescent impurity can interfere with some multicolor stochastic optical reconstruction microscopy/photoactivated localization microscopy measurements in live cells and produce subtle artifacts in chemically fixed cells. We additionally describe alternatives to avoid artifacts in super-resolution localization microscopy.
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