ArticleJournal of applied genetics2014
Oligonucleotides replacing the roles of repetitive sequences pAs1, pSc119.2, pTa-535, pTa71, CCS1, and pAWRC.1 for FISH analysis.
Article in Journal of applied genetics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 226 papers.
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226 citing papers in PubMed, 457 citations in OpenAlex.
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- Comparative Analysis of Satellite DNA inPlants (Basel, Switzerland) · 2025Article
- Development and Characterization of Wheat-Plants (Basel, Switzerland) · 2025Article
- Dynamics of repetitive DNA sequences over the course of evolution and intraspecific divergence of tetraploid goat-grass species Aegilops biuncialis vis.BMC plant biology · 2025Article
- New records of powdery mildew fungi on stone fruits from India.Scientific reports · 2025Article
- Adaptation of centromeres to breakage through local genomic and epigenomic remodeling in wheat.Genome research · 2025Article
166 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Hybrids derived from wheat (Triticum aestivum L.) × rye (Secale cereale L.) have been widely studied because of their important roles in wheat cultivar improvement. Repetitive sequences pAs1, pSc119.2, pTa-535, pTa71, CCS1, and pAWRC.1 are usually used as probes in fluorescence in situ hybridization (FISH) analysis of wheat, rye, and hybrids derived from wheat × rye. Usually, some of these repetitive sequences for FISH analysis were needed to be amplified from a bacterial plasmid, extracted from bacterial cells, and labeled by nick translation. Therefore, the conventional procedure of probe preparation using these repetitive sequences is time-consuming and labor-intensive. In this study, some appropriate oligonucleotide probes have been developed which can replace the roles of repetitive sequences pAs1, pSc119.2, pTa-535, pTa71, CCS1, and pAWRC.1 in FISH analysis of wheat, rye, and hybrids derived from wheat × rye. These oligonucleotides can be synthesized easily and cheaply. Therefore, FISH analysis of wheat and hybrids derived from wheat × rye using these oligonucleotide probes becomes easier and more economical.
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