ArticlePloS one2014
Large sex differences in chicken behavior and brain gene expression coincide with few differences in promoter DNA-methylation.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- DNA Methylation Associates With Sex-Specific Effects of Experimentally Increased Yolk Testosterone in Wild Nestlings.Molecular ecology · 2025Article
- The regulation of methylation on the Z chromosome and the identification of multiple novel Male Hyper-Methylated regions in the chicken.PLoS genetics · 2024Article
- From maternal glucocorticoid and thyroid hormones to epigenetic regulation of offspring gene expression: An experimental study in a wild bird species.Evolutionary applications · 2023Article
- Chromatin accessibility, not 5mC methylation covaries with partial dosage compensation in crows.PLoS genetics · 2023Article
- How Inhibitory Control Relates to Positive and Negative Affective States in Red Junglefowl.Frontiers in veterinary science · 2022Article
- Tameness correlates with domestication related traits in a Red Junglefowl intercross.Genes, brain, and behavior · 2021Article
- The Gene Expression Profile of the Song Control Nucleus HVC Shows Sex Specificity, Hormone Responsiveness, and Species Specificity Among Songbirds.Frontiers in neuroscience · 2021Article
- Article
- DNA methylation in canine brains is related to domestication and dog-breed formation.PloS one · 2020Article
- Sexual Dimorphism for Coping Styles Complements Traditional Methods for Sex Determination in a Multivariety Endangered Hen Breed.Animals : an open access journal from MDPI · 2019Article
- Rapid behavioral assay using handling test provides breed and sex differences in tameness of chickens.Brain and behavior · 2019Article
- DNA methylation and expression of proopiomelanocortin (FEBS open bio · 2018Article
- Epigenetics and early domestication: differences in hypothalamic DNA methylation between red junglefowl divergently selected for high or low fear of humans.Genetics, selection, evolution : GSE · 2018Article
- The relationship between learning speed and personality is age- and task-dependent in red junglefowl.Behavioral ecology and sociobiology · 2018Article
- Genetic and Targeted eQTL Mapping Reveals Strong Candidate Genes Modulating the Stress Response During Chicken Domestication.G3 (Bethesda, Md.) · 2017Article
- Measuring Animal Age with DNA Methylation: From Humans to Wild Animals.Frontiers in genetics · 2017Review
- Domestication and tameness: brain gene expression in red junglefowl selected for less fear of humans suggests effects on reproduction and immunology.Royal Society open science · 2016Article
- The chicken erythrocyte epigenome.Epigenetics & chromatin · 2016Article
- Domestication Effects on Stress Induced Steroid Secretion and Adrenal Gene Expression in Chickens.Scientific reports · 2015Article
- Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
While behavioral sex differences have repeatedly been reported across taxa, the underlying epigenetic mechanisms in the brain are mostly lacking. Birds have previously shown to have only limited dosage compensation, leading to high sex bias of Z-chromosome gene expression. In chickens, a male hyper-methylated region (MHM) on the Z-chromosome has been associated with a local type of dosage compensation, but a more detailed characterization of the avian methylome is limiting our interpretations. Here we report an analysis of genome wide sex differences in promoter DNA-methylation and gene expression in the brain of three weeks old chickens, and associated sex differences in behavior of Red Junglefowl (ancestor of domestic chickens). Combining DNA-methylation tiling arrays with gene expression microarrays we show that a specific locus of the MHM region, together with the promoter for the zinc finger RNA binding protein (ZFR) gene on chromosome 1, is strongly associated with sex dimorphism in gene expression. Except for this, we found few differences in promoter DNA-methylation, even though hundreds of genes were robustly differentially expressed across distantly related breeds. Several of the differentially expressed genes are known to affect behavior, and as suggested from their functional annotation, we found that female Red Junglefowl are more explorative and fearful in a range of tests performed throughout their lives. This paper identifies new sites and, with increased resolution, confirms known sites where DNA-methylation seems to affect sexually dimorphic gene expression, but the general lack of this association is noticeable and strengthens the view that birds do not have dosage compensation.
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