ArticleInternational journal of legal medicine2014
The time-dependent expression of α7nAChR during skeletal muscle wound healing in rats.
Article in International journal of legal medicine, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.
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14 citing papers in PubMed, 1 synthesis or guideline pooled it, 28 citations in OpenAlex.
- State-of-the-Art on Wound Vitality Evaluation: A Systematic Review.International journal of molecular sciences · 2022Pooled it
- Antifibrotic Effects of an α7 Nicotinic Acetylcholine Receptor Agonist in Keloid Fibroblasts and a Rat Scar Model.International journal of molecular sciences · 2025Article
- The time-dependent expression of FPR2 and ANXA1 in murine deep vein thrombosis model and its relation to thrombus age.Forensic science, medicine, and pathology · 2024Article
- Article
- Novel ratio-expressions of genes enables estimation of wound age in contused skeletal muscle.International journal of legal medicine · 2024Article
- Detection of multiple biomarkers associated with satellite cell fate in the contused skeletal muscle of rats for wound age estimation.International journal of legal medicine · 2023Article
- Vitality and wound-age estimation in forensic pathology: review and future prospects.Forensic sciences research · 2020Review
- Temporal expression of wound healing-related genes inform wound age estimation in rats after a skeletal muscle contusion: a multivariate statistical model analysis.International journal of legal medicine · 2020Article
- GTS-21 attenuates loss of body mass, muscle mass, and function in rats having systemic inflammation with and without disuse atrophy.Pflugers Archiv : European journal of physiology · 2018Article
- Detection of RAGE expression and its application to diabetic wound age estimation.International journal of legal medicine · 2017Article
- The Protective Effect of Alpha 7 Nicotinic Acetylcholine Receptor Activation on Critical Illness and Its Mechanism.International journal of biological sciences · 2017Review
- Activation of the α7 nicotinic receptor promotes lipopolysaccharide-induced conversion of M1 microglia to M2.American journal of translational research · 2017Article
- Detection of satellite cells during skeletal muscle wound healing in rats: time-dependent expressions of Pax7 and MyoD in relation to wound age.International journal of legal medicine · 2016Article
- α7nAChR is expressed in satellite cells at different myogenic status during skeletal muscle wound healing in rats.Journal of molecular histology · 2015Article
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10 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
The study on time-dependent expression of α7 nicotine acetylcholine receptor (α7nAChR) was performed by immunohistochemistry, Western blotting, and real-time PCR during skeletal muscle wound healing in rats. Furthermore, co-localization of α7nAChR with macrophage or myofibroblast marker was detected by double immunofluorescence. A total of 50 Sprague-Dawley male rats were divided into control and contusion groups (3 h, 6 h, 12 h, 1 day, 3 days, 5 days, 7 days, 10 days, and 14 days post-injury). In the uninjured controls, α7nAChR positive staining was observed in the sarcolemma and sarcoplasm of normal myofibers. In wounded specimens, a small number of polymorphonuclear cells, a number of macrophages and myofibroblasts showed positive reaction for α7nAChR in contused zones. Morphometrically, the average ratios of α7nAChR-positive cells were over 50 % from 3 to 10 days after contusion, and exceeded 60 % at 5 and 7 days post-injury. Besides, the positive ratios of α7nAChR were <50 % at the other posttraumatic intervals. By Western blotting analysis, the average ratio of α7nAChR protein expression maximized at 7 days after injury, which was >2.13. Similarly, the relative quantity of α7nAChR mRNA expression peaked at 7 days post-wounding as compared with control by real-time PCR detection, showing a relative quantity of >2.65. In conclusion, the expression of α7nAChR is upregulated and temporally distributed in macrophages and myofibroblasts during skeletal muscle wound healing, which might be closely involved in inflammatory response and fibrotic repair after injury. Moreover, α7nAChR is promising as a useful marker for wound age determination of skeletal muscle.
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