ArticlemBio2014
Integrative conjugative element ICE-βox confers oxidative stress resistance to Legionella pneumophila in vitro and in macrophages.
Article in mBio, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 30 citations in OpenAlex.
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- Development of heat-shock resistance inApplied and environmental microbiology · 2023Article
- Effector-mediated subversion of proteasome activator (PA)28αβ enhances host defense against Legionella pneumophila under inflammatory and oxidative stress conditions.PLoS pathogens · 2023Article
- Eat or Be Eaten: Strategies Used byInfection and immunity · 2023Review
- A graph-based approach for the visualisation and analysis of bacterial pangenomes.BMC bioinformatics · 2022Article
- Legionnaires' Disease Mortality in Guinea Pigs Involves the p45 Mobile Genomic Element.The Journal of infectious diseases · 2019Article
- Unusual Legionnaires' outbreak in cool, dry Western Canada: an investigation using genomic epidemiology.Epidemiology and infection · 2017Article
- The Legionella pneumophila GIG operon responds to gold and copper in planktonic and biofilm cultures.PloS one · 2017Article
- Active and adaptive Legionella CRISPR-Cas reveals a recurrent challenge to the pathogen.Cellular microbiology · 2016Article
- Legionella pneumophila strain associated with the first evidence of person-to-person transmission of Legionnaires' disease: a unique mosaic genetic backbone.Scientific reports · 2016Article
- csrT Represents a New Class of csrA-Like Regulatory Genes Associated with Integrative Conjugative Elements of Legionella pneumophila.Journal of bacteriology · 2016Article
- csrR, a Paralog and Direct Target of CsrA, Promotes Legionella pneumophila Resilience in Water.mBio · 2015Article
- Complete and ubiquitinated proteome of the Legionella-containing vacuole within human macrophages.Journal of proteome research · 2015Article
- Integrative and Conjugative Elements (ICEs): What They Do and How They Work.Annual review of genetics · 2015Review
- The many forms of a pleomorphic bacterial pathogen-the developmental network of Legionella pneumophila.Frontiers in microbiology · 2014Review
- Article
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
ABSTRACT Integrative conjugative elements (ICEs) are mobile blocks of DNA that can contribute to bacterial evolution by self-directed transmission of advantageous traits. Here, we analyze the activity of a putative 65-kb ICE harbored by Legionella pneumophila using molecular genetics, conjugation assays, a phenotype microarray screen, and macrophage infections. The element transferred to a naive L. pneumophila strain, integrated site-specifically, and conferred increased resistance to oxacillin, penicillin, hydrogen peroxide, and bleach. Furthermore, the element increased survival of L. pneumophila within restrictive mouse macrophages. In particular, this ICE protects L. pneumophila from phagocyte oxidase activity, since mutation of the macrophage NADPH oxidase eliminated the fitness difference between strains that carried and those that lacked the mobile element. Renamed ICE-βox (for β-lactam antibiotics and oxidative stress), this transposable element is predicted to contribute to the emergence of L. pneumophila strains that are more fit in natural and engineered water systems and in macrophages. IMPORTANCE Bacteria evolve rapidly by acquiring new traits via horizontal gene transfer. Integrative conjugative elements (ICEs) are mobile blocks of DNA that encode the machinery necessary to spread among bacterial populations. ICEs transfer antibiotic resistance and other bacterial survival factors as cargo genes carried within the element. Here, we show that Legionella pneumophila, the causative agent of Legionnaires' disease, carries ICE-βox, which enhances the resistance of this opportunistic pathogen to bleach and β-lactam antibiotics. Moreover, L. pneumophila strains encoding ICE-βox are more resistant to macrophages that carry phagocyte oxidase. Accordingly, ICE-βox is predicted to increase the fitness of L. pneumophila in natural and engineered waters and in humans. To our knowledge, this is the first description of an ICE that confers oxidative stress resistance to a nosocomial pathogen.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.