Evidence map›Paper›PMID 24780883›Full record

ArticleVirulence2014

Comparative analysis of virulence and resistance profiles of Salmonella Enteritidis isolates from poultry meat and foodborne outbreaks in northern Jordan.

Ziad W Jaradat, Leena Abedel Hafiz, Mustafa M Ababneh, Qotaibah O Ababneh, Waseem Al Mousa, Anas Al-Nabulsi, Tareq M Osaili, Richard Holley

Open access · goldAbstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Virulence, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed, 1 pooled it
–field-weighted citation impact, top 86% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 1 synthesis or guideline pooled it, 9 citations in OpenAlex.

  1. Antimicrobial-resistant foodborne pathogens in the Middle East: a systematic review.Environmental science and pollution research international · 2021
    Pooled it
  2. Article
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 3 countries.

Ziad W JaradatDepartment of Biotechnology and Genetic Engineering; Jordan University of Science and Technology; Irbid, Jordan.
Leena Abedel HafizDepartment of Biotechnology and Genetic Engineering; Jordan University of Science and Technology; Irbid, Jordan.
Mustafa M AbabnehDepartment of Basic Veterinary Medical Sciences; Jordan University of Science and Technology; Irbid, Jordan.
Qotaibah O AbabnehDepartment of Biotechnology and Genetic Engineering; Jordan University of Science and Technology; Irbid, Jordan; Department of Biochemistry and Biophysics; Texas A&M University; College Station, TX USA.
Waseem Al MousaDepartment of Biotechnology and Genetic Engineering; Jordan University of Science and Technology; Irbid, Jordan.
Anas Al-NabulsiDepartment of Nutrition and Food Technology; Jordan University of Science and Technology; Irbid, Jordan.
Tareq M OsailiDepartment of Nutrition and Food Technology; Jordan University of Science and Technology; Irbid, Jordan.
Richard HolleyDepartment of Food Science; University of Manitoba; Winnipeg, MB Canada.
Jordan University of Science and Technology · JOCollege Station Medical Center · USUniversity of Manitoba · CA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study was conducted to isolate Salmonella Enteritidis from poultry samples and compare their virulence and antibiotic resistance profiles to S. Enteritidis isolated from outbreaks in northern Jordan. Two hundred presumptive isolates were obtained from 302 raw poultry samples and were subjected to further analysis and confirmation. A phylogenic tree based on 16S rRNA sequencing was constructed and selected isolates representing each cluster were further studied for their virulence in normal adult Swiss white mice. The most virulent strains were isolated from poultry samples and had an LD 50 of 1.55 × 10 (5) CFU, while some of the outbreak isolates were avirulent in mice. Antibiotic resistance profiling revealed that the isolates were resistant to seven of eight antibiotics screened with each isolate resistant to multiple antibiotics (from two to six). Of the poultry isolates, 100%, 88.9%, 77.8%, 66.7%, and 50% showed resistance to nalidixic acid, ciprofloxacin, ampicillin, cephalothin, and cefoperazone, respectively. Two outbreak isolates were sensitive to all tested antibiotics, while 71.4% were resistant to cefoperazone and only 28.6% showed resistance to nalidixic acid. Salmonella outbreak isolates were genetically related to poultry isolates as inferred from the 16S rRNA sequencing, yet were phenotypically different. Although outbreak strains were similar to poultry isolates, when tested in the mouse model, some of the outbreak isolates were highly virulent while others were avirulent. This might be due to a variation in susceptibility of the mouse to different S. Enteritidis isolates.

Indexed as

AnimalsAnti-Bacterial AgentsCluster AnalysisDisease OutbreaksDNA, BacterialDNA, RibosomalDrug Resistance, BacterialFoodborne DiseasesGenotypeHumansJordanLethal Dose 50MeatMiceMicrobial Sensitivity TestsPhylogenyAnti-Bacterial AgentsDNA, BacterialDNA, RibosomalRNA, Ribosomal, 16SVirulence Factorsantimicrobial susceptibilityLD50PCR detectionpoultrySalmonellaEnteritidisvirulence

Identifiers

PMID24780883
PMCPMC4105309
OpenAlexW1982382062

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.