ArticleDevelopmental biology2014
Foxi3 is necessary for the induction of the chick otic placode in response to FGF signaling.
Article in Developmental biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 44 citations in OpenAlex.
- Lipid-mediated reinforcement of FGF/MAPK signaling enables robust otic placode specification.bioRxiv : the preprint server for biology · 2026Article
- Genetics and Epigenetics in the Genesis and Development of Microtia.The Journal of craniofacial surgery · 2024Article
- In vitro modeling of cranial placode differentiation: Recent advances, challenges, and perspectives.Developmental biology · 2024Review
- Sensory cells in tunicates: insights into mechanoreceptor evolution.Frontiers in cell and developmental biology · 2024Review
- The Foxi3 transcription factor is necessary for the fate restriction of placodal lineages at the neural plate border.Development (Cambridge, England) · 2023Article
- The spontaneous mouse mutant low set ears (Lse) is caused by tandem duplication of Fgf3 and Fgf4.Mammalian genome : official journal of the International Mammalian Genome Society · 2023Article
- Article
- Transcriptome analysis reveals an Atoh1b-dependent gene set downstream of Dlx3b/4b during early inner ear development in zebrafish.Biology open · 2023Article
- Comparative assessment of Fgf's diverse roles in inner ear development: A zebrafish perspective.Developmental dynamics : an official publication of the American Association of Anatomists · 2021Review
- Building the Border: Development of the Chordate Neural Plate Border Region and Its Derivatives.Frontiers in physiology · 2020Review
- Insights Into the Early Gene Regulatory Network Controlling Neural Crest and Placode Fate Choices at the Neural Border.Frontiers in physiology · 2020Review
- Enhancer activation by FGF signalling during otic induction.Developmental biology · 2020Article
- Tbx1 and Foxi3 genetically interact in the pharyngeal pouch endoderm in a mouse model for 22q11.2 deletion syndrome.PLoS genetics · 2019Article
- Expression of Protein-Coding Gene Orthologs in Zebrafish and Mouse Inner Ear Non-sensory Supporting Cells.Frontiers in neuroscience · 2019Article
- In Ovo Electroporation of Plasmid DNA and Morpholinos into Specific Tissues During Early Embryogenesis.Methods in molecular biology (Clifton, N.J.) · 2019Article
- Foxi3 transcription factor activity is mediated by a C-terminal transactivation domain and regulated by the Protein Phosphatase 2A (PP2A) complex.Scientific reports · 2018Article
- A Review ofFrontiers in cell and developmental biology · 2018Article
- Gene, cell, and organ multiplication drives inner ear evolution.Developmental biology · 2017Review
- Dlx3b/4b is required for early-born but not later-forming sensory hair cells during zebrafish inner ear development.Biology open · 2017Article
- Cell interactions, signals and transcriptional hierarchy governing placode progenitor induction.Development (Cambridge, England) · 2017Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Vertebrate cranial sensory organs are derived from region at the border of the anterior neural plate called the pre-placodal region (PPR). The otic placode, the anlagen of the inner ear, is induced from PPR ectoderm by FGF signaling. We have previously shown that competence of embryonic ectoderm to respond to FGF signaling during otic placode induction correlates with the expression of PPR genes, but the molecular basis of this competence is poorly understood. Here, we characterize the function of a transcription factor, Foxi3 that is expressed at very early stages in the non-neural ectoderm and later in the PPR of chick embryos. Ablation experiments showed that the underlying hypoblast is necessary for the initiation of Foxi3 expression. Mis-expression of Foxi3 was sufficient to induce markers of non-neural ectoderm such as Dlx5, and the PPR such as Six1 and Eya2. Electroporation of Dlx5, or Six1 together with Eya1 also induced Foxi3, suggesting direct or indirect positive regulation between non-neural ectoderm genes and PPR genes. Knockdown of Foxi3 in chick embryos prevented the induction of otic placode markers, and was able to prevent competent cranial ectoderm from expressing otic markers in response to FGF2. In contrast, Foxi3 expression alone was not sufficient to confer competence to respond to FGF on embryonic ectoderm. Our analysis of PPR and FGF-responsive genes after Foxi3 knockdown at gastrula stages suggests it is not necessary for the expression of PPR genes at these stages, nor for the transduction of FGF signals. The early expression but late requirement for Foxi3 in ear induction suggests it may have some of the properties associated with pioneer transcription factors.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.