Evidence map›Paper›PMID 24780398›Full record

ArticleBiochemical and biophysical research communications2014

Enhanced prostacyclin formation and Wnt signaling in sclerostin deficient osteocytes and bone.

Zachary C Ryan, Theodore A Craig, Jeffrey L Salisbury, Lomeli R Carpio, Meghan McGee-Lawrence, Jennifer J Westendorf, Rajiv Kumar

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biochemical and biophysical research communications, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.4field-weighted citation impact, top 36% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. Lung tumor cells inhibit bone mineralization and osteoblast activity.Biochemical and biophysical research communications · 2019
    Article
  3. Mechanical loading disrupts osteocyte plasma membranes which initiates mechanosensation events in bone.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2018
    Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Zachary C RyanDivision of Nephrology and Hypertension, Department of Medicine, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA.
Theodore A CraigDivision of Nephrology and Hypertension, Department of Medicine, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA.
Jeffrey L SalisburyDepartment of Biochemistry and Molecular Biology, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA.
Lomeli R CarpioMayo Graduate School, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA.
Meghan McGee-LawrenceDepartment of Orthopedic Surgery, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA.
Jennifer J WestendorfDepartment of Biochemistry and Molecular Biology, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA; Department of Orthopedic Surgery, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA.
Rajiv KumarDivision of Nephrology and Hypertension, Department of Medicine, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA; Department of Biochemistry and Molecular Biology, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA. Electronic address: rkumar@mayo.edu.
Mayo Clinic · US

Funding

Musculoskeletal Research Training ProgramT32AR056950 · NIAMS · MAYO CLINIC ROCHESTER · PI Jennifer J Westendorf · 2009 to 2026
$7.0M
Runx2 and Axin2 Interactions During Bone FormationR01DE020194 · NIDCR · MAYO CLINIC ROCHESTER · PI WESTENDORF, JENNIFER J · 2010 to 2014
$2.1M
Structure of Sclerostin Protein ComplexesR21AR060869 · NIAMS · MAYO CLINIC ROCHESTER · PI KUMAR, RAJIV · 2011 to 2012
$390k
Relationship between Hdac3 suppression and Wnt signaling in osteoblastsF32AR060140 · NIAMS · MAYO CLINIC ROCHESTER · PI MCGEE-LAWRENCE, MEGHAN E. · 2010 to 2011
$99k
NIAMS NIH HHS AR60869NIAMS NIH HHS F32 AR060140NIAMS NIH HHS F32 AR60140NIAMS NIH HHS R21 AR060869NIAMS NIH HHS T32 AR056950NIDCR NIH HHS R01 DE020194
6 · The paper itself

Abstract

We show that prostacyclin production is increased in bone and osteocytes from sclerostin (Sost) knockout mice which have greatly increased bone mass. The addition of prostacyclin or a prostacyclin analog to bone forming osteoblasts enhances differentiation and matrix mineralization of osteoblasts. The increase in prostacyclin synthesis is linked to increases in β-catenin concentrations and activity as shown by enhanced binding of lymphoid enhancer factor, Lef1, to promoter elements within the prostacyclin synthase promoter. Blockade of Wnt signaling reduces prostacyclin production in osteocytes. Increased prostacyclin production by osteocytes from sclerostin deficient mice could potentially contribute to the increased bone formation seen in this condition.

Indexed as

Adaptor Proteins, Signal TransducingAnimalsbeta CateninBone and BonesEpoprostenolGlycoproteinsIntercellular Signaling Peptides and ProteinsLymphoid Enhancer-Binding Factor 1MiceMice, KnockoutOsteocytesWnt Signaling PathwayAdaptor Proteins, Signal Transducingbeta CateninEpoprostenolGlycoproteinsIntercellular Signaling Peptides and ProteinsLef1 protein, mouseLymphoid Enhancer-Binding Factor 1Sost protein, mouseOsteocytesProstacyclinSclerostin deficiencyWnt

Identifiers

PMID24780398
PMCPMC4052706
OpenAlexW2071843886

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.