ArticleProceedings of the National Academy of Sciences of the United States of America2014
Collagen-binding protein, Aegyptin, regulates probing time and blood feeding success in the dengue vector mosquito, Aedes aegypti.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 53 citations in OpenAlex.
- Genetic control of malaria transmission: a salivary gland-centric perspective.Current opinion in insect science · 2026Review
- Chromosome-scale genome assembly and developmental transcriptome of Aedes triseriatus, vector of La Crosse virus.BMC genomics · 2026Article
- Progress in research on mosquito-borne viruses: Understanding infection, transmission, and control.Infectious diseases & immunity · 2026Review
- Unraveling the Molecular Mechanisms of Mosquito Salivary Proteins: New Frontiers in Disease Transmission and Control.Biomolecules · 2025Review
- The human CD47 checkpoint is targeted by an immunosuppressiveScience immunology · 2024Article
- Evaluation ofCold Spring Harbor protocols · 2023Article
- A deeper insight into the sialome of male and female Culex quinquefasciatus mosquitoes.BMC genomics · 2023Article
- The salivary protein Saglin facilitates efficient midgut colonization of Anopheles mosquitoes by malaria parasites.PLoS pathogens · 2023Article
- Mosquito Salivary Proteins and Arbovirus Infection: From Viral Enhancers to Potential Targets for Vaccines.Pathogens (Basel, Switzerland) · 2023Review
- Guianensin, aFrontiers in immunology · 2023Article
- Performing Immunohistochemistry in Mosquito Salivary Glands.Cold Spring Harbor protocols · 2022Article
- A deeper insight into the sialome of male and female Ochlerotatus triseriatus mosquitoes.Insect biochemistry and molecular biology · 2022Article
- Article
- Vertebrate Responses against Arthropod Salivary Proteins and Their Therapeutic Potential.Vaccines · 2021Review
- Biochemical characterization of AeD7L2 and its physiological relevance in blood feeding in the dengue mosquito vector, Aedes aegypti.The FEBS journal · 2021Article
- Aedes aegypti post-emergence transcriptome: Unveiling the molecular basis for the hematophagic and gonotrophic capacitation.PLoS neglected tropical diseases · 2021Article
- AeMOPE-1, a Novel Salivary Peptide FromFrontiers in immunology · 2021Article
- The structures of two salivary proteins from the West Nile vectorCurrent research in structural biology · 2021Article
- ADP binding by the Culex quinquefasciatus mosquito D7 salivary protein enhances blood feeding on mammals.Nature communications · 2020Article
- Novel salivary gland allergens from tropical mosquito species and IgE reactivity in allergic patients.The World Allergy Organization journal · 2020Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Mosquito salivary glands have important roles in blood feeding and pathogen transmission. However, the biological relevance of many salivary components has yet to be determined. Aegyptin, a secreted salivary protein from Aedes aegypti, binds collagen and inhibits platelet aggregation and adhesion. We used a transgenic approach to study the relevance of Aegyptin in mosquito blood feeding. Aedes aegypti manipulated genetically to express gene-specific inverted-repeat RNA sequences exhibited significant reductions in Aegyptin mRNA accumulation (85-87%) and protein levels (>80-fold) in female mosquito salivary glands. Transgenic mosquitoes had longer probing times (78-300 s, P < 0.0001) when feeding on mice compared with controls (15-56 s), feeding success was reduced, and those feeding took smaller blood meals. However, no differences in feeding success or blood meal size were found in membrane feeding experiments using defibrinated human blood. Salivary gland extracts from transgenic mosquitoes failed to inhibit collagen-induced platelet aggregation in vitro. Reductions of Aegyptin did not affect salivary ADP-induced platelet aggregation inhibition or disturb anticlotting activities. Our results demonstrate the relevance of Aegyptin for A. aegypti blood feeding, providing further support for the hypothesis that platelet aggregation inhibition is a vital salivary function in blood feeding arthropods. It has been suggested that the multiple mosquito salivary components mediating platelet aggregation (i.e., Aegyptin, apyrase, D7) represent functional redundancy. Our findings do not support this hypothesis; instead, they indicate that multiple salivary components work synergistically and are necessary to achieve maximum blood feeding efficiency.
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