ArticleThe Journal of biological chemistry2014
α-SNAP interferes with the zippering of the SNARE protein membrane fusion machinery.
Article in The Journal of biological chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- A pathogenic mutation in α-SNAP impairs membrane lipid binding by concealing a critical hydrophobic loop.Communications biology · 2026Article
- CaScience advances · 2025Article
- Fluorescence Anisotropy for Monitoring cis- and trans-Membrane Interactions of Synaptotagmin-1.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Photosensitive Nanoprobes for Rapid Isolation and Size-Specific Enrichment of Synthetic and Extracellular Vesicle Subpopulations.Advanced functional materials · 2024Article
- Requirement of Cholesterol for Calcium-Dependent Vesicle Fusion by Strengthening Synaptotagmin-1-Induced Membrane Bending.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Alpha-SNAP (M105I) mutation promotes neuronal differentiation of neural stem/progenitor cells through overactivation of AMPK.Frontiers in cell and developmental biology · 2023Article
- Molecular Mechanisms Underlying Neurotransmitter Release.Annual review of biophysics · 2022Review
- Selected tools to visualize membrane interactions.European biophysics journal : EBJ · 2021Review
- Isolation of large dense-core vesicles from bovine adrenal medulla for functional studies.Scientific reports · 2020Article
- Munc18-1 is crucial to overcome the inhibition of synaptic vesicle fusion by αSNAP.Nature communications · 2019Article
- Multiple factors maintain assembled trans-SNARE complexes in the presence of NSF and αSNAP.eLife · 2019Article
- Single-Molecule Optical Tweezers Study of Regulated SNARE Assembly.Methods in molecular biology (Clifton, N.J.) · 2019Article
- Mechanism of neurotransmitter release coming into focus.Protein science : a publication of the Protein Society · 2018Review
- The heptad repeat domain 1 of Mitofusin has membrane destabilization function in mitochondrial fusion.EMBO reports · 2018Article
- Arrest ofThe Journal of biological chemistry · 2018Article
- Sec17 (α-SNAP) and an SM-tethering complex regulate the outcome of SNARE zippering in vitro and in vivo.eLife · 2017Article
- Energetics, kinetics, and pathway of SNARE folding and assembly revealed by optical tweezers.Protein science : a publication of the Protein Society · 2017Review
- An activated Q-SNARE/SM protein complex as a possible intermediate in SNARE assembly.The EMBO journal · 2017Article
- Gasotransmitter Heterocellular Signaling.Antioxidants & redox signaling · 2017Article
- NaeLife · 2017Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Neuronal exocytosis is mediated by soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins. Before fusion, SNARE proteins form complexes bridging the membrane followed by assembly toward the C-terminal membrane anchors, thus initiating membrane fusion. After fusion, the SNARE complex is disassembled by the AAA-ATPase N-ethylmaleimide-sensitive factor that requires the cofactor α-SNAP to first bind to the assembled SNARE complex. Using chromaffin granules and liposomes we now show that α-SNAP on its own interferes with the zippering of membrane-anchored SNARE complexes midway through the zippering reaction, arresting SNAREs in a partially assembled trans-complex and preventing fusion. Intriguingly, the interference does not result in an inhibitory effect on synaptic vesicles, suggesting that membrane properties also influence the final outcome of α-SNAP interference with SNARE zippering. We suggest that binding of α-SNAP to the SNARE complex affects the ability of the SNARE complex to harness energy or transmit force to the membrane.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.