ArticleThe Journal of biological chemistry2014
Cyclin-dependent kinase 8 module expression profiling reveals requirement of mediator subunits 12 and 13 for transcription of Serpent-dependent innate immunity genes in Drosophila.
Article in The Journal of biological chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 20 citations in OpenAlex.
- Subunits Med12 and Med13 of Mediator Cooperate with Subunits SAYP and Bap170 of SWI/SNF in Active Transcription inInternational journal of molecular sciences · 2024Article
- A Co-Association Network Analysis Reveals Putative Regulators for Health-Related Traits in Pigs.Frontiers in immunology · 2021Article
- Mediator complex subunit Med19 binds directly GATA transcription factors and is required with Med1 for GATA-driven gene regulationThe Journal of biological chemistry · 2020Article
- Differential Requirements for Mediator Complex Subunits inFrontiers in immunology · 2020Article
- The subunit assembly state of the Mediator complex is nutrient-regulated and is dysregulated in a genetic model of insulin resistance and obesity.The Journal of biological chemistry · 2019Article
- Article
- Understanding Obesity as a Risk Factor for Uterine Tumors Using Drosophila.Advances in experimental medicine and biology · 2019Review
- Article
- Depletion of Mediator Kinase Module Subunits Represses Superenhancer-Associated Genes in Colon Cancer Cells.Molecular and cellular biology · 2018Article
- Mediator complex component MED13 regulates zygotic genome activation and is required for postimplantation development in the mouse.Biology of reproduction · 2018Article
- Commentary:Frontiers in immunology · 2018Article
- BET Bromodomain Inhibition Releases the Mediator Complex from Select cis-Regulatory Elements.Cell reports · 2016Article
- The Mediator complex: a central integrator of transcription.Nature reviews. Molecular cell biology · 2015Review
- The Arabidopsis Mediator CDK8 module genes CCT (MED12) and GCT (MED13) are global regulators of developmental phase transitions.Development (Cambridge, England) · 2014Article
- Heart- and muscle-derived signaling system dependent on MED13 and Wingless controls obesity in Drosophila.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Cdk8 (cyclin-dependent kinase 8) module of Mediator integrates regulatory cues from transcription factors to RNA polymerase II. It consists of four subunits where Med12 and Med13 link Cdk8 and cyclin C (CycC) to core Mediator. Here we have investigated the contributions of the Cdk8 module subunits to transcriptional regulation using RNA interference in Drosophila cells. Genome-wide expression profiling demonstrated separation of Cdk8-CycC and Med12-Med13 profiles. However, transcriptional regulation by Cdk8-CycC was dependent on Med12-Med13. This observation also revealed that Cdk8-CycC and Med12-Med13 often have opposite transcriptional effects. Interestingly, Med12 and Med13 profiles overlapped significantly with that of the GATA factor Serpent. Accordingly, mutational analyses indicated that GATA sites are required for Med12-Med13 regulation of Serpent-dependent genes. Med12 and Med13 were also found to be required for Serpent-activated innate immunity genes in defense to bacterial infection. The results reveal a novel role for the Cdk8 module in Serpent-dependent transcription and innate immunity.
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