ArticleInfection and immunity2014
Origins of a 350-kilobase genomic duplication in Mycobacterium tuberculosis and its impact on virulence.
Article in Infection and immunity, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 30 citations in OpenAlex.
- Adaptation of the Mycobacterium tuberculosis transcriptome to biofilm growth.PLoS pathogens · 2024Article
- The chosen few:Frontiers in immunology · 2024Review
- Characterizing in vivo loss of virulence of an HN878 Mycobacterium tuberculosis isolate from a genetic duplication event.Tuberculosis (Edinburgh, Scotland) · 2022Article
- Article
- Dynamics of Extensive Drug Resistance Evolution of Mycobacterium tuberculosis in a Single Patient During 9 Years of Disease and Treatment.The Journal of infectious diseases · 2022Article
- Towards comprehensive understanding of bacterial genetic diversity: large-scale amplifications inMicrobial genomics · 2022Article
- The SNAP hypothesis: Chromosomal rearrangements could emerge from positive Selection during Niche Adaptation.PLoS genetics · 2020Article
- Effects of Host, Sample, andFrontiers in genetics · 2019Article
- Utilisation of the Prestwick Chemical Library to identify drugs that inhibit the growth of mycobacteria.PloS one · 2019Article
- Reference set of Mycobacterium tuberculosis clinical strains: A tool for research and product development.PloS one · 2019Article
- Validation of Novel Mycobacterium tuberculosis Isoniazid Resistance Mutations Not Detectable by Common Molecular Tests.Antimicrobial agents and chemotherapy · 2018Article
- TnSeq of Mycobacterium tuberculosis clinical isolates reveals strain-specific antibiotic liabilities.PLoS pathogens · 2018Article
- Immunological consequences of strain variation within the Mycobacterium tuberculosis complex.European journal of immunology · 2017Review
- Unique Regulation of the DosR Regulon in the Beijing Lineage of Mycobacterium tuberculosis.Journal of bacteriology · 2017Article
- Essential but Not Vulnerable: Indazole Sulfonamides Targeting Inosine Monophosphate Dehydrogenase as Potential Leads against Mycobacterium tuberculosis.ACS infectious diseases · 2017Article
- Respiratory flexibility in response to inhibition of cytochrome C oxidase in Mycobacterium tuberculosis.Antimicrobial agents and chemotherapy · 2014Article
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
In the present study, we have investigated the evolution and impact on virulence of a 350-kb genomic duplication present in the most recently evolved members of the Mycobacterium tuberculosis East Asian lineage. In a mouse model of infection, comparing HN878 subclones HN878-27 (no duplication) and HN878-45 (with the 350-kb duplication) revealed that the latter is impaired for in vivo growth during the initial 3 weeks of infection. Furthermore, the median survival time of mice infected with isolate HN878-45 is significantly longer (77 days) than that of mice infected with HN878-27. Whole-genome sequencing of both isolates failed to reveal any mutational events other than the duplication that could account for such a substantial difference in virulence. Although we and others had previously speculated that the 350-kb duplication arose in response to some form of host-applied selective pressure (P. Domenech, G. S. Kolly, L. Leon-Solis, A. Fallow, M. B. Reed, J. Bacteriol. 192: 4562-4570, 2010, and B. Weiner, J. Gomez, T. C. Victor, R. M. Warren, A. Sloutsky, B. B. Plikaytis, J. E. Posey, P. D. van Helden, N. C. Gey van Pittius, M. Koehrsen, P. Sisk, C. Stolte, J. White, S. Gagneux, B. Birren, D. Hung, M. Murray, J. Galagan, PLoS One 7: e26038, 2012), here we show that this large chromosomal amplification event is very rapidly selected within standard in vitro broth cultures in a range of isolates. Indeed, subclones harboring the duplication were detectable after just five rounds of in vitro passage. In contrast, the duplication appears to be highly unstable in vivo and is negatively selected during the later stages of infection in mice. We believe that the rapid in vitro evolution of M. tuberculosis is an underappreciated aspect of its biology that is often ignored, despite the fact that it has the potential to confound the data and conclusions arising from comparative studies of isolates at both the genotypic and phenotypic levels.
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