Evidence map›Paper›PMID 24776804›Full record

ArticleNature2014

Human oocytes reprogram adult somatic nuclei of a type 1 diabetic to diploid pluripotent stem cells.

Mitsutoshi Yamada, Bjarki Johannesson, Ido Sagi, Lisa Cole Burnett, Daniel H Kort, Robert W Prosser, Daniel Paull, Michael W Nestor, Matthew Freeby, Ellen Greenberg and 6 more

Abstract read
PubMed Publisher
In one paragraph

Article in Nature, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 87 papers.

0numbers the graph read from it
0cells of the map it votes in
87citing papers in PubMed
14.4field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

87 citing papers in PubMed, 189 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Review
  5. Article
  6. Artificial oocyte activation using CaFrontiers in endocrinology · 2023
    Article
  7. Review
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  9. Article
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  12. Observational
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  14. Article
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27 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors at 3 institutions in 2 countries.

Mitsutoshi Yamada1] The New York Stem Cell Foundation Research Institute, New York, New York 10032, USA [2].
Bjarki Johannesson1] The New York Stem Cell Foundation Research Institute, New York, New York 10032, USA [2].
Ido SagiStem Cell Unit, Department of Genetics, Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.
Lisa Cole BurnettNaomi Berrie Diabetes Center, Department of Pediatrics, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA.
Daniel H Kort1] Center for Women's Reproductive Care, College of Physicians and Surgeons, Columbia University, New York 10019, USA [2] Department of Obstetrics and Gynecology, College of Physicians and Surgeons, Columbia University, New York 10032, USA.
Robert W Prosser1] Center for Women's Reproductive Care, College of Physicians and Surgeons, Columbia University, New York 10019, USA [2] Department of Obstetrics and Gynecology, College of Physicians and Surgeons, Columbia University, New York 10032, USA.
Daniel PaullThe New York Stem Cell Foundation Research Institute, New York, New York 10032, USA.
Michael W NestorThe New York Stem Cell Foundation Research Institute, New York, New York 10032, USA.
Matthew FreebyNaomi Berrie Diabetes Center, Department of Pediatrics, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA.
Ellen GreenbergNaomi Berrie Diabetes Center, Department of Pediatrics, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA.
Robin S GolandNaomi Berrie Diabetes Center, Department of Pediatrics, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA.
Rudolph L LeibelNaomi Berrie Diabetes Center, Department of Pediatrics, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA.
Susan L SolomonThe New York Stem Cell Foundation Research Institute, New York, New York 10032, USA.
Nissim BenvenistyStem Cell Unit, Department of Genetics, Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.
Mark V Sauer1] Center for Women's Reproductive Care, College of Physicians and Surgeons, Columbia University, New York 10019, USA [2] Department of Obstetrics and Gynecology, College of Physicians and Surgeons, Columbia University, New York 10032, USA.
Dieter EgliThe New York Stem Cell Foundation Research Institute, New York, New York 10032, USA.
Columbia University · USNew York Stem Cell Foundation · USHebrew University of Jerusalem · IL

Funding

NUTRITIONT32DK007647 · NIDDK · COLUMBIA UNIV NEW YORK MORNINGSIDE · PI Anthony W Ferrante, Lori M Zeltser · 1990 to 2026
$7.4M
NIDDK NIH HHS T32 DK007647
6 · The paper itself

Abstract

The transfer of somatic cell nuclei into oocytes can give rise to pluripotent stem cells that are consistently equivalent to embryonic stem cells, holding promise for autologous cell replacement therapy. Although methods to induce pluripotent stem cells from somatic cells by transcription factors are widely used in basic research, numerous differences between induced pluripotent stem cells and embryonic stem cells have been reported, potentially affecting their clinical use. Because of the therapeutic potential of diploid embryonic stem-cell lines derived from adult cells of diseased human subjects, we have systematically investigated the parameters affecting efficiency of blastocyst development and stem-cell derivation. Here we show that improvements to the oocyte activation protocol, including the use of both kinase and translation inhibitors, and cell culture in the presence of histone deacetylase inhibitors, promote development to the blastocyst stage. Developmental efficiency varied between oocyte donors, and was inversely related to the number of days of hormonal stimulation required for oocyte maturation, whereas the daily dose of gonadotropin or the total number of metaphase II oocytes retrieved did not affect developmental outcome. Because the use of concentrated Sendai virus for cell fusion induced an increase in intracellular calcium concentration, causing premature oocyte activation, we used diluted Sendai virus in calcium-free medium. Using this modified nuclear transfer protocol, we derived diploid pluripotent stem-cell lines from somatic cells of a newborn and, for the first time, an adult, a female with type 1 diabetes.

Indexed as

Cellular ReprogrammingDiploidyAdultBlastocystCell FusionCell NucleusChromosomes, MammalianDiabetes Mellitus, Type 1FemaleHistone Deacetylase InhibitorsHumansInfant, NewbornMetaphaseOocytesOogenesisPluripotent Stem CellsHistone Deacetylase Inhibitors

Identifiers

PMID24776804
OpenAlexW2027669322

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.