Evidence map›Paper›PMID 24775810›Full record

ArticleVirology journal2014

A loop-mediated isothermal amplification assay for the visual detection of duck circovirus.

Liji Xie, Zhixun Xie, Guangyuan Zhao, Jiabo Liu, Yaoshan Pang, Xianwen Deng, Zhiqin Xie, Qing Fan, Sisi Luo

Open access · goldAbstract readEvaluation Study
In one paragraph

Article in Virology journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
0.9field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 29 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Review
  6. Quantitative Loop-Mediated Isothermal Amplification Detection ofInternational journal of molecular sciences · 2023
    Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. One-Day Molecular Detection ofFoods (Basel, Switzerland) · 2021
    Article
  12. Colorimetric biosensors for point-of-care virus detections.Materials science for energy technologies · 2020
    Review
  13. Article
  14. Article
  15. Article
  16. Article
  17. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Liji Xie
Zhixun XieDepartment of Biotechnology, Guangxi Key Laboratory of Animal Vaccines and Diagnostics, Guangxi Veterinary Research Institute, 51 Youai North Road, 530001 Nanning, China. xiezhixun@126.com.
Guangyuan Zhao
Jiabo Liu
Yaoshan Pang
Xianwen Deng
Zhiqin Xie
Qing Fan
Sisi Luo
Guangxi Veterinary Research Institute · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundDuck circovirus (DuCV) infection in farmed ducks is associated with growth problems or retardation syndromes. Rapid identification of DuCV infected ducks is essential to control DuCV effectively. Therefore, this study aims to develop of an assay for DuCV to be highly specific, sensitive, and simple without any specialized equipment.

methodsA set of six specific primers was designed to target the sequences of the Rep gene of DuCV, and A loop-mediated isothermal amplification (LAMP) assay were developed and the reaction conditions were optimized for rapid detection of DuCV.

resultsThe LAMP assay reaction was conducted in a 62°C water bath condition for 50 min. Then the amplification products were visualized directly for color changes. This LAMP assay is highly sensitive and able to detect twenty copies of DuCV DNA. The specificity of this LAMP assay was supported by no cross-reaction with other duck pathogens.

conclusionThis LAMP method for DuCV is highly specific and sensitive and can be used as a rapid and direct diagnostic assay for testing clinical samples.

Indexed as

AnimalsCircoviridae InfectionsCircovirusColorDNA PrimersDNA, ViralDucksNucleic Acid Amplification TechniquesPoultry DiseasesSensitivity and SpecificityTemperatureTimeDNA PrimersDNA, Viral

Identifiers

PMID24775810
PMCPMC4013541
OpenAlexW2156815830

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.