ArticleBMC microbiology2014
Delineation of immunodominant and cytadherence segment(s) of Mycoplasma pneumoniae P1 gene.
Article in BMC microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 20 citations in OpenAlex.
- Immune dysregulation inFrontiers in immunology · 2026Review
- Unravelling distinct patterns of metagenomic surveillance and respiratory microbiota between two P1 genotypes ofEmerging microbes & infections · 2025Article
- Protective immunity induced by a novel P1 adhesin C-terminal anchored mRNA vaccine againstMicrobiology spectrum · 2025Article
- Investigating the occurrence of autoimmune diseases among children and adolescents hospitalized forFrontiers in immunology · 2023Article
- Immunodominant proteins P1 and P40/P90 from human pathogen Mycoplasma pneumoniae.Nature communications · 2020Article
- Serological diagnosis of Mycoplasma pneumoniae infection by using the mimic epitopes.World journal of microbiology & biotechnology · 2018Article
- Pathogenesis and Treatment of Neurologic Diseases Associated WithFrontiers in microbiology · 2018Review
- Pediatric clinical features ofExperimental and therapeutic medicine · 2017Article
- Insights into the pathogenesis of Mycoplasma pneumoniae (Review).Molecular medicine reports · 2016Review
- Article
- Antibody Response to Mycoplasma pneumoniae: Protection of Host and Influence on Outbreaks?Frontiers in microbiology · 2016Review
- Cyto-adherence of Mycoplasma mycoides subsp. mycoides to bovine lung epithelial cells.BMC veterinary research · 2015Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAdhesion of Mycoplasma pneumoniae (M. pneumoniae) to host epithelial cells requires several adhesin proteins like P1, P30 and P116. Among these proteins, P1 protein has been inedited as one of the major adhesin and immunogenic protein present on the attachment organelle of M. pneumoniae. In the present study, we scanned the entire sequence of M. pneumoniae P1 protein to identify the immunodominant and cytadherence region(s). M. pneumoniae P1 gene was synthesized in four segments replacing all the UGA codons to UGG codons. Each of the four purified P1 protein fragment was analyzed for its immunogenicity with anti-M. pneumoniae M129 antibodies (Pab M129) and sera of M. pneumoniae infected patients by western blotting and ELISA. Antibodies were produced against all the P1 protein fragments and these antibodies were used for M. pneumoniae adhesion, M. pneumoniae adhesion inhibition and M. pneumoniae surface exposure assays using HEp-2 cells lines.
resultsOur results show that the immunodominant regions are distributed throughout the entire length of P1 protein, while only the N- and C- terminal region(s) of P1 protein are surface exposed and block cytadhesion to HEp-2 cells, while antibodies to two middle fragments failed to block cytadhesion.
conclusionsThese results have important implications in designing strategies to block the attachment of M. pneumoniae to epithelial cells, thus preventing the development of atypical pneumonia.
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