ArticleThe Journal of cell biology1989
Cell adhesion to fibronectin and tenascin: quantitative measurements of initial binding and subsequent strengthening response.
Article in The Journal of cell biology, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 82 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
82 citing papers in PubMed, 414 citations in OpenAlex.
- Integrin Regulated Autoimmune Disorders: Understanding the Role of Mechanical Force in Autoimmunity.Frontiers in cell and developmental biology · 2022Review
- TGF-β triggers rapid fibrillogenesis via a novel TβRII-dependent fibronectin-trafficking mechanism.Molecular biology of the cell · 2017Article
- Harvesting pre-polarized macrophages using thermo-responsive substrates.Scientific reports · 2017Article
- Cooperative effects of fibronectin matrix assembly and initial cell-substrate adhesion strength in cellular self-assembly.Acta biomaterialia · 2016Article
- Biopolymers and supramolecular polymers as biomaterials for biomedical applications.MRS bulletin · 2015Article
- The effect of five proteins on stem cells used for osteoblast differentiation and proliferation: a current review of the literature.Cellular and molecular life sciences : CMLS · 2014Review
- Article
- Dosage of amyloid precursor protein affects axonal contact guidance in Down syndrome.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2014Article
- Enteric neural crest-derived cells promote their migration by modifying their microenvironment through tenascin-C production.Developmental biology · 2013Article
- Amyloid precursor protein is an autonomous growth cone adhesion molecule engaged in contact guidance.PloS one · 2013Article
- Biocompatibility of Graphene Oxide.Nanoscale research letters · 2011Article
- The role of the microenvironment in tumor growth and invasion.Progress in biophysics and molecular biology · 2011Review
- Nucleus pulposus cell-matrix interactions with laminins.European cells & materials · 2011Article
- Tenascins and the importance of adhesion modulation.Cold Spring Harbor perspectives in biology · 2011Review
- The influence of bisphosphonates on human osteoblast migration and integrin aVb3/tenascin C gene expression in vitro.Head & face medicine · 2011Article
- New insights into vinculin function and regulation.International review of cell and molecular biology · 2011Review
- Contractility modulates cell adhesion strengthening through focal adhesion kinase and assembly of vinculin-containing focal adhesions.Journal of cellular physiology · 2010Article
- Quantitative analysis of three-dimensional human mammary epithelial tissue architecture reveals a role for tenascin-C in regulating c-met function.The American journal of pathology · 2010Article
- The changing paradigm of outflow resistance generation: towards synergistic models of the JCT and inner wall endothelium.Experimental eye research · 2009Review
- VASP involvement in force-mediated adherens junction strengthening.Biochemical and biophysical research communications · 2008Article
22 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Cell-substratum adhesion strengths have been quantified using fibroblasts and glioma cells binding to two extracellular matrix proteins, fibronectin and tenascin. A centrifugal force-based adhesion assay was used for the adhesive strength measurements, and the corresponding morphology of the adhesions was visualized by interference reflection microscopy. The initial adhesions as measured at 4 degrees C were on the order of 10(-5)dynes/cell and did not involve the cytoskeleton. Adhesion to fibronectin after 15 min at 37 degrees C were more than an order of magnitude stronger; the strengthening response required cytoskeletal involvement. By contrast to the marked strengthening of adhesion to FN, adhesion to TN was unchanged or weakened after 15 min at 37 degrees C. The absolute strength of adhesion achieved varied according to protein and cell type. When a mixed substratum of fibronectin and tenascin was tested, the presence of tenascin was found to reduce the level of the strengthening of cell adhesion normally observed at 37 degrees C on a substratum of fibronectin alone. Parallel analysis of corresponding interference reflection micrographs showed that differences in the area of cell surface within 10-15 nm of the substratum correlated closely with each of the changes in adhesion observed: after incubation for 15 min on fibronectin at 37 degrees C, glioma cells increased their surface area within close contact to the substrate by integral to 125-fold. Cells on tenascin did not increase their surface area of contact. The increased surface area of contact and the inhibitory activity of cytochalasin b suggest that the adhesive "strengthening" in the 15 min after initial binding brings additional adhesion molecules into the adhesive site and couples the actin cytoskeleton to the adhesion complex.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.