ArticleFrontiers in behavioral neuroscience2014
Recombineering strategies for developing next generation BAC transgenic tools for optogenetics and beyond.
Article in Frontiers in behavioral neuroscience, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- A sex-specific effect of MFrontiers in molecular neuroscience · 2024Article
- Generating endogenous Myh11-driven Cre mice for sex-independent gene deletion in smooth muscle cells.JCI insight · 2023Article
- Improving the Efficacy and Accessibility of Intracranial Viral Vector Delivery in Non-Human Primates.Pharmaceutics · 2022Article
- Article
- BAC Recombineering and Transgenesis to Study Cell Polarity and Polarized Tissue Morphogenesis in Mice.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Altered baseline and amphetamine-mediated behavioral profiles in dopamine transporter Cre (DAT-Ires-Cre) mice compared to tyrosine hydroxylase Cre (TH-Cre) mice.Psychopharmacology · 2020Article
- Probing Nicotinic Acetylcholine Receptor Function in Mouse Brain Slices via Laser Flash Photolysis of Photoactivatable Nicotine.Journal of visualized experiments : JoVE · 2019Article
- Characterization of a BAC transgenic mouse expressing Krt19-driven iCre recombinase in its digestive organs.PloS one · 2019Article
- Two eARCHT3.0 Lines for Optogenetic Silencing of Dopaminergic and Serotonergic Neurons.Frontiers in neural circuits · 2019Article
- Electrophysiological properties of medium spiny neurons in the nucleus accumbens core of prepubertal male and female Drd1a-tdTomato line 6 BAC transgenic mice.Journal of neurophysiology · 2018Article
- Altered Baseline and Nicotine-Mediated Behavioral and Cholinergic Profiles in ChAT-Cre Mouse Lines.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2018Article
- Optogenetics: the new molecular approach to control functions of neural cells in epilepsy, depression and tumors of the central nervous system.American journal of cancer research · 2018Review
- The Expanding Toolkit of Translating Ribosome Affinity Purification.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
- Characterization of Optically and Electrically Evoked Dopamine Release in Striatal Slices from Digenic Knock-in Mice with DAT-Driven Expression of Channelrhodopsin.ACS chemical neuroscience · 2017Article
- Dopamine D1 receptor expression is bipolar cell type-specific in the mouse retina.The Journal of comparative neurology · 2016Article
- Transgenic labeling of parvalbumin-expressing neurons with tdTomato.Neuroscience · 2016Article
- VAChT overexpression increases acetylcholine at the synaptic cleft and accelerates aging of neuromuscular junctions.Skeletal muscle · 2016Article
- Intrinsic Cholinergic Neurons in the Hippocampus: Fact or Artifact?Frontiers in synaptic neuroscience · 2016Article
- Shining light on motivation, emotion, and memory processes.Frontiers in behavioral neuroscience · 2015Article
- Modern and simple construction of plasmid: saving time and cost.Journal of microbiology (Seoul, Korea) · 2014Review
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2 authors.
Funding
Abstract
The development and application of diverse BAC transgenic rodent lines has enabled rapid progress for precise molecular targeting of genetically-defined cell types in the mammalian central nervous system. These transgenic tools have played a central role in the optogenetic revolution in neuroscience. Indeed, an overwhelming proportion of studies in this field have made use of BAC transgenic Cre driver lines to achieve targeted expression of optogenetic probes in the brain. In addition, several BAC transgenic mouse lines have been established for direct cell-type specific expression of Channelrhodopsin-2 (ChR2). While the benefits of these new tools largely outweigh any accompanying challenges, many available BAC transgenic lines may suffer from confounds due in part to increased gene dosage of one or more "extra" genes contained within the large BAC DNA sequences. Here we discuss this under-appreciated issue and propose strategies for developing the next generation of BAC transgenic lines that are devoid of extra genes. Furthermore, we provide evidence that these strategies are simple, reproducible, and do not disrupt the intended cell-type specific transgene expression patterns for several distinct BAC clones. These strategies may be widely implemented for improved BAC transgenesis across diverse disciplines.
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