ArticleThe Journal of infectious diseases2014
Novel genotyping tools for investigating transmission dynamics of Plasmodium falciparum.
Article in The Journal of infectious diseases, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 2 of them syntheses that pooled it.
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Who cites it
29 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Prevalence and associated factors of malaria among the displaced population in refugee camps in Africa: a systematic review and meta-analysis.Malaria journal · 2025Pooled it
- Malaria prevalence in Mauritania: a systematic review and meta-analysis.Malaria journal · 2023Pooled it
- Risk factor for gametocyte carriage and gametocytemia in Plasmodium vivax and Plasmodium falciparum.Infectious diseases of poverty · 2025Article
- Genotyping Plasmodium falciparum gametocytes using amplicon deep sequencing.Malaria journal · 2024Article
- Genetic complexity alters drug susceptibility of asexual and gametocyte stages ofAntimicrobial agents and chemotherapy · 2024Article
- Plasmodium falciparum gametocyte carriage in longitudinally monitored incident infections is associated with duration of infection and human host factors.Scientific reports · 2023Article
- Biology and epidemiology ofParasite epidemiology and control · 2023Review
- Persistent Plasmodium falciparum infections enhance transmission-reducing immunity development.Scientific reports · 2021Article
- Plasmodium falciparum gametocyte carriage in symptomatic patients shows significant association with genetically diverse infections, anaemia, and asexual stage density.Malaria journal · 2021Article
- Immunity against sexual stage Plasmodium falciparum and Plasmodium vivax parasites.Immunological reviews · 2020Review
- Prevalence of asymptomaticWellcome open research · 2020Article
- Genetic profiling of the Plasmodium falciparum parasite population in uncomplicated malaria from India.Malaria journal · 2019Article
- Malaria Molecular Epidemiology: An Evolutionary Genetics Perspective.Microbiology spectrum · 2019Review
- Article
- Transmission of molecularly undetectable circulating parasite clones leads to high infection complexity in mosquitoes post feeding.International journal for parasitology · 2018Article
- Plasmodium Gametocytes in Field Studies: Do We Measure Commitment to Transmission or Detectability?Trends in parasitology · 2018Review
- Article
- Detection of Plasmodium falciparum male and female gametocytes and determination of parasite sex ratio in human endemic populations by novel, cheap and robust RTqPCR assays.Malaria journal · 2017Article
- Plasmodium vivax genetic diversity and heterozygosity in blood samples and resulting oocysts at the Thai-Myanmar border.Malaria journal · 2017Article
- A Direct from Blood Reverse Transcriptase Polymerase Chain Reaction Assay for Monitoring Falciparum Malaria Parasite Transmission in Elimination Settings.The American journal of tropical medicine and hygiene · 2017Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
backgroundDifferentiation between gametocyte-producing Plasmodium falciparum clones depends on both high levels of stage-specific transcripts and high genetic diversity of the selected genotyping marker obtained by a high-resolution typing method. By analyzing consecutive samples of one host, the contribution of each infecting clone to transmission and the dynamics of gametocyte production in multiclone infections can be studied.
methodsWe have evaluated capillary electrophoresis based differentiation of 6 length-polymorphic gametocyte genes. RNA and DNA of 25 µL whole blood from 46 individuals from Burkina Faso were simultaneously genotyped.
resultsHighest discrimination power was achieved by pfs230 with 18 alleles, followed by pfg377 with 15 alleles. When assays were performed in parallel on RNA and DNA, 85.7% of all pfs230 samples and 59.5% of all pfg377 samples contained at least one matching genotype in DNA and RNA.
conclusionsThe imperfect detection in both, DNA and RNA, was identified as major limitation for investigating transmission dynamics, owing primarily to the volume of blood processed and the incomplete representation of all clones in the sample tested. Abundant low-density gametocyte carriers impede clone detectability, which may be improved by analyzing larger volumes and detecting initially sequestered gametocyte clones in follow-up samples.
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