ArticlePflugers Archiv : European journal of physiology2015
Extracellular H+ induces Ca2+ signals in respiratory chemoreceptors of zebrafish.
Article in Pflugers Archiv : European journal of physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed, 39 citations in OpenAlex.
- Serotonin stimulates proliferation of ionocytes via the 5-HT2A receptor in zebrafish larvae.The Journal of experimental biology · 2026Article
- Oxygen chemoreceptor inhibition by dopamine DThe Journal of physiology · 2025Article
- Insights into the control and consequences of breathing adjustments in fishes-from larvae to adults.Frontiers in physiology · 2023Review
- Single-cell transcriptomic analysis of neuroepithelial cells and other cell types of the gills of zebrafish (Danio rerio) exposed to hypoxia.Scientific reports · 2022Article
- The influence of environmental calcium on the branchial morphology in a catadromous fish.Environmental science and pollution research international · 2021Article
- A marine teleost, Opsanus beta, compensates acidosis in hypersaline water by HJournal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2021Article
- The role of TASK-2 channels in COAmerican journal of physiology. Regulatory, integrative and comparative physiology · 2020Article
- Characterization of ion channels and OJournal of neurophysiology · 2017Article
- Lactate provides a strong pH-independent ventilatory signal in the facultative air-breathing teleost Pangasianodon hypophthalmus.Scientific reports · 2017Article
- Role of endogenous carbon monoxide in the control of breathing in zebrafish (Danio rerio).American journal of physiology. Regulatory, integrative and comparative physiology · 2016Article
- An emerging role for gasotransmitters in the control of breathing and ionic regulation in fish.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2016Review
- Purinergic and Cholinergic Drugs Mediate Hyperventilation in Zebrafish: Evidence from a Novel Chemical Screen.PloS one · 2016Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Neuroepithelial cells (NECs) of the fish gill are respiratory chemoreceptors that detect changes in O2 and CO2/H(+) and are homologous to type I cells of the mammalian carotid body. In zebrafish (Danio rerio), stimulation of NECs by hypoxia or hypercapnia initiates inhibition of K(+) channels and subsequent membrane depolarisation. The goal of the present study was to further elucidate, in zebrafish NECs, the signalling pathways that underlie CO2/H(+) sensing and generate intracellular Ca(2+) ([Ca(2+)]i) signals. Breathing frequency was elevated maximally in fish exposed to 5 % CO2 (~37.5 mmHg). Measurement of [Ca(2+)]i in isolated NECs using Fura-2 imaging indicated that [Ca(2+)]i increased in response to acidic hypercapnia (5 % CO2, pH 6.6) and isocapnic acidosis (normocapnia, pH 6.6), but not to isohydric hypercapnia (5 % CO2, pH 7.6). Measurement of intracellular pH (pHi) using BCECF demonstrated a rapid decrease in pHi in response to acidic and isohydric hypercapnia, while isocapnic acidosis produced a smaller change in pHi. Intracellular acidification was reduced by the carbonic anhydrase inhibitor, acetazolamide, without affecting [Ca(2+)]i responses. Moreover, intracellular acidification using acetate (at constant extracellular pH) was without effect on [Ca(2+)]i. The acid-induced increase in [Ca(2+)]i persisted in the absence of extracellular Ca(2+) and was unaffected by Ca(2+) channel blockers (Cd(2+), Ni(2+) or nifedipine). The results of this study demonstrate that, unlike type I cells, extracellular H(+) is critical to the hypercapnia-induced increase in [Ca(2+)]i in NECs. The increase in [Ca(2+)]i occurs independently of pHi and appears to originate primarily from Ca(2+) derived from intracellular stores.
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