ArticleThe Plant cell2014
Methylcrotonyl-CoA Carboxylase Regulates Triacylglycerol Accumulation in the Model Diatom Phaeodactylum tricornutum.
Article in The Plant cell, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
45 citing papers in PubMed, 127 citations in OpenAlex.
- Heterologous expression of AtLEC1 and AtLEC1-LIKE transcription factors redirects carbon flux toward lipid accumulation in diatom.Microbial cell factories · 2025Article
- Optimizing Phaeodactylum tricornutum cultivation: integrated strategies for enhancing biomass, lipid, and fucoxanthin production.Biotechnology for biofuels and bioproducts · 2025Article
- Optimizing Nitrate and Tryptone to Enhance Growth and Triacylglycerol Accumulation inJournal of microbiology and biotechnology · 2024Article
- Presence of vitamin BPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Article
- Evolution of the regulatory subunits for the heteromeric acetyl-CoA carboxylase.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Review
- Improving Lipid Content in the DiatomCurrent issues in molecular biology · 2024Article
- Genomic and transcriptome analyses reveal potential contributors to erucic acid biosynthesis in seeds of rapeseed (Brassica napus).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- Strategies of NaCl Tolerance in Saline-Alkali-Tolerant Green MicroalgaPlants (Basel, Switzerland) · 2023Article
- Chlamydomonas mutants lacking chloroplast TRIOSE PHOSPHATE TRANSPORTER3 are metabolically compromised and light sensitive.The Plant cell · 2023Article
- PDAT regulates PE as transient carbon sink alternative to triacylglycerol in Nannochloropsis.Plant physiology · 2022Article
- The relationship between amino acid and lipid metabolism in oleaginous eukaryotic microorganism.Applied microbiology and biotechnology · 2022Review
- The Transition Toward Nitrogen Deprivation in Diatoms Requires Chloroplast Stand-By and Deep Metabolic Reshuffling.Frontiers in plant science · 2021Article
- Amino Acid Catabolism During Nitrogen Limitation inFrontiers in plant science · 2020Review
- Targeted Metabolomic and Biochemical Changes During Nitrogen Stress Mediated Lipid Accumulation inFrontiers in bioengineering and biotechnology · 2020Article
- Bioengineering of Microalgae: Recent Advances, Perspectives, and Regulatory Challenges for Industrial Application.Frontiers in bioengineering and biotechnology · 2020Review
- Stresses as First-Line Tools for Enhancing Lipid and Carotenoid Production in Microalgae.Frontiers in bioengineering and biotechnology · 2020Review
- Time-resolved multi-omics analysis reveals the role of nutrient stress-induced resource reallocation for TAG accumulation in oleaginous fungusBiotechnology for biofuels · 2020Article
- Transcriptome Changes Induced by Different Potassium Levels in Banana Roots.Plants (Basel, Switzerland) · 2019Article
- Elevated COMicrobial cell factories · 2019Article
- Metabolic Innovations Underpinning the Origin and Diversification of the Diatom Chloroplast.Biomolecules · 2019Review
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The model marine diatom Phaeodactylum tricornutum can accumulate high levels of triacylglycerols (TAGs) under nitrogen depletion and has attracted increasing attention as a potential system for biofuel production. However, the molecular mechanisms involved in TAG accumulation in diatoms are largely unknown. Here, we employed a label-free quantitative proteomics approach to estimate differences in protein abundance before and after TAG accumulation. We identified a total of 1193 proteins, 258 of which were significantly altered during TAG accumulation. Data analysis revealed major changes in proteins involved in branched-chain amino acid (BCAA) catabolic processes, glycolysis, and lipid metabolic processes. Subsequent quantitative RT-PCR and protein gel blot analysis confirmed that four genes associated with BCAA degradation were significantly upregulated at both the mRNA and protein levels during TAG accumulation. The most significantly upregulated gene, encoding the β-subunit of methylcrotonyl-CoA carboxylase (MCC2), was selected for further functional studies. Inhibition of MCC2 expression by RNA interference disturbed the flux of carbon (mainly in the form of leucine) toward BCAA degradation, resulting in decreased TAG accumulation. MCC2 inhibition also gave rise to incomplete utilization of nitrogen, thus lowering biomass during the stationary growth phase. These findings help elucidate the molecular and metabolic mechanisms leading to increased lipid production in diatoms.
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Registered trials
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