ArticleMetabolic engineering2014
Comparative engineering of Escherichia coli for cellobiose utilization: Hydrolysis versus phosphorolysis.
Article in Metabolic engineering, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
8 citing papers in PubMed.
- Unlocking raw-corncob consolidated bioprocessing for malic acid production by DyP-enabled lignin deconstruction inSynthetic and systems biotechnology · 2027Article
- Advances, challenges, and future directions towards a cellulolytic Escherichia coli.Biotechnology for biofuels and bioproducts · 2026Review
- Enhanced glycolic acid yield through xylose and cellobiose utilization by metabolically engineered Escherichia coli.Bioprocess and biosystems engineering · 2021Article
- Simultaneous consumption of cellobiose and xylose byBiotechnology for biofuels · 2018Article
- Biochemical properties of GH94 cellodextrin phosphorylase THA_1941 from a thermophilic eubacterium Thermosipho africanus TCF52B with cellobiose phosphorylase activity.Scientific reports · 2017Article
- Metabolic engineering of Agrobacterium sp. ATCC31749 for curdlan production from cellobiose.Journal of industrial microbiology & biotechnology · 2016Article
- A paradigm shift in biomass technology from complete to partial cellulose hydrolysis: lessons learned from nature.Bioengineered · 2015Article
- A constitutive expression system for cellulase secretion in Escherichia coli and its use in bioethanol production.PloS one · 2015Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microbial biocatalysts capable of cellobiose assimilation are of interest in bioconversion of cellulosic materials. This study provides a careful comparison in the two mechanisms of cellobiose assimilation, hydrolysis versus phosphorolysis, between two otherwise isogenic E. coli strains. Relative to cells assimilating cellobiose hydrolytically, phosphorolysis cells tolerated common inhibitors better under both anaerobic and aerobic conditions. Additionally, phosphorolysis cells were able to direct the favorable energy metabolism to recombinant protein production, resulting in up to five fold more recombinant proteins. In a mixed sugar fermentation (5% (w/v) cellobiose+5.0% (w/v) xylose), however, xylose utilization in phosphorolysis cells came to a complete halt after only about 60% consumption whereas the hydrolysis cells were able to ferment both sugars to near completion. These results provide insights into the new metabolic engineering strategy. To our best knowledge, this is the first comparison study in E. coli on the two cellobiose assimilation mechanisms.
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24769131What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.