Evidence map›Paper›PMID 24769114›Full record

ArticleActa biomaterialia2014

Cardiac differentiation of cardiosphere-derived cells in scaffolds mimicking morphology of the cardiac extracellular matrix.

Yanyi Xu, Sourav Patnaik, Xiaolei Guo, Zhenqing Li, Wilson Lo, Ryan Butler, Andrew Claude, Zhenguo Liu, Ge Zhang, Jun Liao and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Acta biomaterialia, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.

0numbers the graph read from it
0cells of the map it votes in
27citing papers in PubMed
8.2field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

27 citing papers in PubMed, 52 citations in OpenAlex.

  1. Review
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  14. Biomimetic polyurethane/TiOMaterials science & engineering. C, Materials for biological applications · 2018
    Article
  15. Injectable Bioengineered Hydrogel Therapy in the Treatment of Ischemic Cardiomyopathy.Current treatment options in cardiovascular medicine · 2017
    Review
  16. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 4 institutions in 1 country.

Yanyi XuDepartment of Materials Science and Engineering, The Ohio State University, Columbus, OH 43210, USA.
Sourav PatnaikDepartment of Agricultural & Biological Engineering, Mississippi State University, Starkville, MS 39672, USA.
Xiaolei GuoDepartment of Materials Science and Engineering, The Ohio State University, Columbus, OH 43210, USA.
Zhenqing LiDepartment of Materials Science and Engineering, The Ohio State University, Columbus, OH 43210, USA.
Wilson LoDepartment of Biochemistry, The Ohio State University, Columbus, OH 43210, USA.
Ryan ButlerDepartment of Clinical Science, Mississippi State University, Starkville, MS 39672, USA.
Andrew ClaudeDepartment of Clinical Science, Mississippi State University, Starkville, MS 39672, USA.
Zhenguo LiuDavis Heart and Lung Research Institute, The Ohio State University, Columbus, OH 43210, USA.
Ge ZhangDepartment of Biomedical Engineering, University of Akron, Akron, OH 44325, USA.
Jun LiaoDepartment of Agricultural & Biological Engineering, Mississippi State University, Starkville, MS 39672, USA.
Peter M AndersonDepartment of Materials Science and Engineering, The Ohio State University, Columbus, OH 43210, USA.
Jianjun GuanDepartment of Materials Science and Engineering, The Ohio State University, Columbus, OH 43210, USA. Electronic address: guan.21@osu.edu.
The Ohio State University · USMississippi State University · USLung Institute · USUniversity of Akron · US

Funding

MG53-Mediated Membrane Repair in Muscle Physiology and DiseaseR01AR061385 · NIAMS · UNIV OF MED/DENT NJ-R W JOHNSON MED SCH · PI LIN, PEI-HUI · 2011 to 2020
$3.8M
Targeting cell membrane repair for treatment of acute kidney injuryR01DK106394 · NIDDK · OHIO STATE UNIVERSITY · PI LIN, PEI-HUI, ROVIN, BRAD H · 2016 to 2020
$2.5M
Hydrogel encapsulation of a tissue repair protein to treat chronic woundsR01AG056919 · NIA · OHIO STATE UNIVERSITY · PI GUAN, JIANJUN, LI, HAICHANG · 2017 to 2021
$2.4M
Myokine function of MG53 in muscle injury-repair and regenerationR01AR070752 · NIAMS · OHIO STATE UNIVERSITY · PI HAN, RENZHI · 2017 to 2021
$1.7M
POLYMERIC ELECTRON PARAMAGNETIC RESONANCE PROBES FOR REAL-TIME MONITORING OF TISSUE VASCULARIZATIONR21EB021896 · NIBIB · WASHINGTON UNIVERSITY · PI GUAN, JIANJUN · 2016 to 2017
$413k
NIAMS NIH HHS R01 AR061385NIAMS NIH HHS R01 AR070752NIA NIH HHS R01 AG056919NIDDK NIH HHS R01 DK106394
6 · The paper itself

Abstract

Stem cell therapy has the potential to regenerate heart tissue after myocardial infarction (MI). The regeneration is dependent upon cardiac differentiation of the delivered stem cells. We hypothesized that timing of the stem cell delivery determines the extent of cardiac differentiation as cell differentiation is dependent on matrix properties such as biomechanics, structure and morphology, and these properties in cardiac extracellular matrix (ECM) continuously vary with time after MI. In order to elucidate the relationship between ECM properties and cardiac differentiation, we created an in vitro model based on ECM-mimicking fibers and a type of cardiac progenitor cell, cardiosphere-derived cells (CDCs). A simultaneous fiber electrospinning and cell electrospraying technique was utilized to fabricate constructs. By blending a highly soft hydrogel with a relatively stiff polyurethane and modulating fabrication parameters, tissue constructs with similar cell adhesion property but different global modulus, single fiber modulus, fiber density and fiber alignment were achieved. The CDCs remained alive within the constructs during a 1week culture period. CDC cardiac differentiation was dependent on the scaffold modulus, fiber volume fraction and fiber alignment. Two constructs with relatively low scaffold modulus, ∼50-60kPa, most significantly directed the CDC differentiation into mature cardiomyocytes as evidenced by gene expressions of cardiac troponin T (cTnT), calcium channel (CACNA1c) and cardiac myosin heavy chain (MYH6), and protein expressions of cardiac troponin I (cTnI) and connexin 43 (CX43). Of these two low-modulus constructs, the extent of differentiation was greater for lower fiber alignment and higher fiber volume fraction. These results suggest that cardiac ECM properties may have an effect on cardiac differentiation of delivered stem cells.

Indexed as

AnimalsBiomimetic MaterialsCell DifferentiationCell ProliferationCells, CulturedExtracellular MatrixHydrogelsMiceMyocytes, CardiacSpheroids, CellularStem CellsTissue EngineeringHydrogelsCardiac differentiationCardiosphere-derived cellsMatrix modulusStem cell therapy

Identifiers

PMID24769114
PMCPMC6029687
OpenAlexW1968203242

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.