ArticleNeuron2014
Chemogenetic synaptic silencing of neural circuits localizes a hypothalamus→midbrain pathway for feeding behavior.
Article in Neuron, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 258 papers.
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Who cites it
258 citing papers in PubMed.
- Immature olfactory sensory neurons provide complementary input in the intact olfactory system.iScience · 2026Article
- Differential contributions of striatal dopaminergic pathways to visuomotor conditional learning.Psychopharmacology · 2026Article
- Rapid rescue of despair behaviors by sonogenetic neuromodulation of the mPFC-DRN pathway.Molecular psychiatry · 2026Article
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- Next-generation chemogenetic inhibition using a brain-permeant non-prescription agent.Signal transduction and targeted therapy · 2026Article
- The hindbrain-hypothalamic noradrenergic pathway controls homeostatic food intake but not cue-induced appetitive behaviors.Journal of neuroendocrinology · 2026Article
- Response dynamics of discrete subiculum→retrosplenial cortex projections underlying trace fear conditioning.iScience · 2026Article
- Advances in chemogenetics: a review of DREADDs and its application in psychiatric disorders.Molecular psychiatry · 2026Review
- Immature olfactory sensory neurons provide complementary input in the healthy olfactory system.bioRxiv : the preprint server for biology · 2025Article
- Divergent prefrontal cortex circuits regulate cued food seeking under distinct metabolic or emotional states.Nature communications · 2025Article
- A network of basolateral amygdala projection neurons contributes to stress-induced activation of the hypothalamic-pituitary-adrenal axis.Science advances · 2025Article
- Article
- Glucagon-Like Peptide 1 (GLP-1) Action on Hypothalamic Feeding Circuits.Endocrinology · 2025Review
- Central terminals of primary afferents coordinate the spontaneous activity of dorsal horn neurons.The Journal of physiology · 2025Article
- Astrocytes release ATP/ADP and glutamate in flashes via vesicular exocytosis.Molecular psychiatry · 2025Article
- Somatostatin neurons detect stimulus-reward contingencies to reduce neocortical inhibition during learning.Cell reports · 2025Article
- Refining the role of the paraventricular hypothalamus in feeding behavior.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Article
- Hippocampal Inhibitory Interneuron-Specific DREADDs Treatment Alters mTORC1-4E-BP Signaling and Impairs Memory Formation.Journal of neurochemistry · 2025Article
198 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors.
Funding
Abstract
Brain function is mediated by neural circuit connectivity, and elucidating the role of connections is aided by techniques to block their output. We developed cell-type-selective, reversible synaptic inhibition tools for mammalian neural circuits by leveraging G protein signaling pathways to suppress synaptic vesicle release. Here, we find that the pharmacologically selective designer Gi-protein-coupled receptor hM4D is a presynaptic silencer in the presence of its cognate ligand clozapine-N-oxide (CNO). Activation of hM4D signaling sharply reduced synaptic release probability and synaptic current amplitude. To demonstrate the utility of this tool for neural circuit perturbations, we developed an axon-selective hM4D-neurexin variant and used spatially targeted intracranial CNO injections to localize circuit connections from the hypothalamus to the midbrain responsible for feeding behavior. This synaptic silencing approach is broadly applicable for cell-type-specific and axon projection-selective functional analysis of diverse neural circuits.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.