ArticlePhytochemistry2014
The effect of drought stress on the expression of key genes involved in the biosynthesis of triterpenoid saponins in liquorice (Glycyrrhiza glabra).
Article in Phytochemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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46 citing papers in PubMed, 150 citations in OpenAlex.
- Metabolomics reveal drought-stress responses in guayule, a semi-arid rubber crop.Metabolomics : Official journal of the Metabolomic Society · 2026Article
- Water deficit modulates growth, secondary metabolism, and carvacrol biosynthesis gene expression inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2026Article
- Effects of drought duration on terpene profiles, physiological responses, and terpene-related gene expression in rosemary.BMC plant biology · 2026Article
- Microbial consortia and their metabolic modulations as mechanisms of water deficit tolerance in strawberry plants.Frontiers in plant science · 2026Article
- Effects of drought stress on growth, physiology and bioactive compound accumulation in licorice (Frontiers in plant science · 2026Article
- Exogenous ethanol treatment promotes glycyrrhizin accumulation in aseptically grownPlant signaling & behavior · 2025Article
- Abiotic Stress Alters the Nutritional, Metabolomic, and Glycomic Profiles ofFoods (Basel, Switzerland) · 2025Article
- Enhancing salt tolerance in Mentha × gracilis through foliar applications of titanium and nano-titanium.BMC plant biology · 2025Article
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- Titanium dioxide -mediated regulation of enzymatic and non-enzymatic antioxidants, pigments, and diosgenin content promotes cold stress tolerance in Trigonella foenum-graecum L.Scientific reports · 2025Article
- Induction of stigma-like structures in saffron (Crocus sativus L.): Exploring factors and metabolite analysis.PloS one · 2025Article
- Estimation of the heritability of medicinal compound contents in Glycyrrhiza uralensis.PloS one · 2025Article
- Integrated transcriptome and metabolome analyses reveal key genes regulating jujuboside biosynthesis inFrontiers in plant science · 2025Article
- Identification and analysis of UGT genes associated with triterpenoid saponin in soapberry (Sapindus mukorossi Gaertn.).BMC plant biology · 2024Article
- Rice seeds biofortification using biogenic ıron oxide nanoparticles synthesized by using Glycyrrhiza glabra: a study on growth and yield ımprovement.Scientific reports · 2024Article
- Differential responses of two fenugreek (Trigonella foenum-graecum L.) landraces pretreated with melatonin to prolonged drought stress and subsequent recovery.BMC plant biology · 2024Article
- The interaction effect of water deficit stress and seaweed extract on phytochemical characteristics and antioxidant activity of licorice (Frontiers in plant science · 2024Article
- Multi-Omics Elucidates Difference in Accumulation of Bioactive Constituents in Licorice (Molecules (Basel, Switzerland) · 2023Article
- 4-methylumbelliferone (4-MU) enhances drought tolerance of apple by regulating rhizosphere microbial diversity and root architecture.Horticulture research · 2023Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Glycyrrhiza glabra is an important medicinal plant throughout the world. Glycyrrhizin is a triterpenoid that is among the most important secondary metabolites produced by liquorice. Drought stress is proposed to enhance the levels of secondary metabolites. In this study, the effect of drought stress on the expression of important genes involved in the glycyrrhizin biosynthetic pathway was examined. Drought stress at the seedling stage was applied to 8-day-old plants using polyethylene glycol. Subsequently, the samples were collected 0, 4, 8 or 24 h post-treatment. At the adult plant stage, 10-month-old plants were subjected to drought stress by discontinuing irrigation. Subsequently, samples were collected at 2, 16 and 28 days after drought imposition (S(2d), S(16d) and S(28d), respectively). We performed semi-quantitative RT-PCR assays to evaluate the gene expression levels of sequalene synthase (SQS), β-amyrin synthase (bAS), lupeol synthase (LUS) and cycloartenol synthase (CAS) during stress. Finally, the glycyrrhizin content of stolons was determined via HPLC. The results revealed that due to osmotic stress, the gene expression levels of SQS and bAS were increased, whereas those of CAS were relatively unchanged at the seedling stage. At the adult plant stage, the expression levels of SQS and bAS were increased under drought stress conditions, whereas the gene expression level of CAS remained relatively constant. The glycyrrhizin content in stolons was increased only under severe drought stress conditions (S(28d)). Our results indicate that application of controlled drought stress up-regulates the expression of key genes involved in the biosynthesis of triterpenoid saponins and directly enhances the production of secondary metabolites, including glycyrrhizin, in liquorice plants.
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