ArticleBMC microbiology2014
Detection and quantification of Flavobacterium psychrophilum in water and fish tissue samples by quantitative real time PCR.
Article in BMC microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 46 citations in OpenAlex.
- Identification and Characterization of Flavobacterium psychrophilum as a Causative Pathogen in Farmed Grass Carp Ctenopharyngodon idella in China.Journal of fish diseases · 2026Article
- Article
- Environmental DNA/RNA for non-invasive early detection and monitoring of pathogen dynamics in Atlantic salmon (Aquaculture (Amsterdam, Netherlands) · 2026Article
- A novel high-throughput qPCR chip for solving co-infections in RAS farmed rainbow trout.Scientific reports · 2024Article
- The Plant-Associated Flavobacterium: A Hidden Helper for Improving Plant Health.The plant pathology journal · 2024Review
- VaryingMicrobiology spectrum · 2024Article
- Impact of Infection withPathogens (Basel, Switzerland) · 2023Article
- Protective Effect of [Cu(NNMicroorganisms · 2022Article
- FELASA-AALAS Recommendations for Monitoring and Reporting of Laboratory Fish Diseases and Health Status, with an Emphasis on Zebrafish (Comparative medicine · 2022Article
- Detecting Flavobacterial Fish Pathogens in the Environment via High-Throughput Community Analysis.Applied and environmental microbiology · 2022Article
- Bacterial cold-water disease in ayu (Frontiers in cellular and infection microbiology · 2022Article
- Phenotypic and Genetic Characterization of Flavobacterium psychrophilum Recovered from Diseased Salmonids in China.Microbiology spectrum · 2021Article
- Oral Administration ofFrontiers in immunology · 2021Article
- An eDNA/eRNA-based approach to investigate the life cycle of non-cultivable shellfish micro-parasites: the case of Bonamia ostreae, a parasite of the European flat oyster Ostrea edulis.Microbial biotechnology · 2020Article
- Validation of a quantitative PCR assay for the detection of 2Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2020Article
- Development of a Quantitative PCR Assay for Arcobacter spp. and its Application to Environmental Water Samples.Microbes and environments · 2018Article
- Stress Tolerance-Related Genetic Traits of Fish PathogenFrontiers in microbiology · 2018Article
- More Than Gliding: Involvement of GldD and GldG in the Virulence ofFrontiers in microbiology · 2017Article
- Lack of a type-2 glycosyltransferase in the fish pathogen Flavobacterium psychrophilum determines pleiotropic changes and loss of virulence.Veterinary research · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundFlavobacterium psychrophilum is the agent of Bacterial Cold Water Disease and Rainbow Trout Fry Syndrome, two diseases leading to high mortality. Pathogen detection is mainly carried out using cultures and more rapid and sensitive methods are needed.
resultsWe describe a qPCR technique based on the single copy gene β' DNA-dependent RNA polymerase (rpoC). Its detection limit was 20 gene copies and the quantification limit 103 gene copies per reaction. Tests on spiked spleens with known concentrations of F. psychrophilum (106 to 101 cells per reaction) showed no cross-reactions between the spleen tissue and the primers and probe. Screening of water samples and spleens from symptomless and infected fishes indicated that the pathogen was already present before the outbreaks, but F. psychrophilum was only quantifiable in spleens from diseased fishes.
conclusionsThis qPCR can be used as a highly sensitive and specific method to detect F. psychrophilum in different sample types without the need for culturing. qPCR allows a reliable detection and quantification of F. psychrophilum in samples with low pathogen densities. Quantitative data on F. psychrophilum abundance could be useful to investigate risk factors linked to infections and also as early warning system prior to potential devastating outbreak.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.