ArticleMolecular and cellular biology1989
Isolation, sequence, and expression of a human keratin K5 gene: transcriptional regulation of keratins and insights into pairwise control.
Article in Molecular and cellular biology, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 93 citations in OpenAlex.
- Biodegradable Gelatin Microcarriers Facilitate Re-Epithelialization of Human Cutaneous Wounds - An In Vitro Study in Human Skin.PloS one · 2015Article
- Keratin 5 expression in squamocellular carcinoma of the head and neck.Oncology letters · 2014Article
- Directed expression of a chimeric type II keratin partially rescues keratin 5-null mice.The Journal of biological chemistry · 2014Article
- Constitutive expression of human keratin 14 gene in mouse lung induces premalignant lesions and squamous differentiation.Carcinogenesis · 2008Article
- Intermediate filaments: a historical perspective.Experimental cell research · 2007Review
- A small surface hydrophobic stripe in the coiled-coil domain of type I keratins mediates tetramer stability.The Journal of cell biology · 2005Article
- Defining the properties of the nonhelical tail domain in type II keratin 5: insight from a bullous disease-causing mutation.Molecular biology of the cell · 2005Article
- Keratin 17 null mice exhibit age- and strain-dependent alopecia.Genes & development · 2002Article
- Formation of a normal epidermis supported by increased stability of keratins 5 and 14 in keratin 10 null mice.Molecular biology of the cell · 2001Article
- Complete cytolysis and neonatal lethality in keratin 5 knockout mice reveal its fundamental role in skin integrity and in epidermolysis bullosa simplex.Molecular biology of the cell · 2001Article
- Introducing a null mutation in the mouse K6alpha and K6beta genes reveals their essential structural role in the oral mucosa.The Journal of cell biology · 2000Article
- The functional diversity of epidermal keratins revealed by the partial rescue of the keratin 14 null phenotype by keratin 16.The Journal of cell biology · 1999Article
- Functional differences between keratins of stratified and simple epithelia.The Journal of cell biology · 1998Article
- Directed expression of keratin 16 to the progenitor basal cells of transgenic mouse skin delays skin maturation.The Journal of cell biology · 1998Article
- Defining the interactions between intermediate filaments and desmosomes.The Journal of cell biology · 1998Article
- Transgenic studies with a keratin promoter-driven growth hormone transgene: prospects for gene therapy.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- The genetic basis of epidermolysis bullosa simplex with mottled pigmentation.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- Onset of re-epithelialization after skin injury correlates with a reorganization of keratin filaments in wound edge keratinocytes: defining a potential role for keratin 16.The Journal of cell biology · 1996Article
- The basal keratin network of stratified squamous epithelia: defining K15 function in the absence of K14.The Journal of cell biology · 1995Article
- A 5'-upstream region of a bovine keratin 6 gene confers tissue-specific expression and hyperproliferation-related induction in transgenic mice.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mitotically active basal layers of most stratified squamous epithelia express 10 to 30% of their total protein as keratin. The two keratins specifically expressed in these cells are the type II keratin K5 (58 kilodaltons) and its corresponding partner, type I keratin K14 (50 kilodaltons), both of which are essential for the formation of 8-nm filaments. Dissecting the molecular mechanisms underlying the coordinate regulation of the two keratins is an important first step in understanding epidermal differentiation and in designing promoters that will enable delivery and expression of foreign gene products in stratified squamous epithelia, e.g., skin. Previously, we reported the sequence of the gene encoding human K14 (D. Marchuk, S. McCrohon, and E. Fuchs, Cell 39:491-498, 1984; Marchuk et al., Proc. Natl. Acad. Sci. USA 82:1609-1613, 1985). We have now isolated and characterized the gene encoding human K5. The sequence of the coding portion of this gene matched perfectly with that of a partial K5 cDNA sequence obtained from a cultured human epidermal library (R. Lersch and E. Fuchs, Mol. Cell. Biol. 8:486-493, 1988), and gene transfection studies indicated that the gene is functional. Nuclear runoff experiments demonstrated that the K5 and K14 genes were both transcribed at dramatically higher levels in cultured human epidermal cells than in fibroblasts, indicating that at least part of the regulation of the expression of this keratin pair is at the transcriptional level. When the K5 gene was transfected transiently into NIH 3T3 fibroblasts, foreign expression of the gene caused the appearance of endogenous mouse K14 and the subsequent formation of a keratin filament array in the cells. In this case, transcriptional changes did not appear to be involved in the regulation, suggesting that there may be multiple control mechanisms underlying the pairwise expression of keratins.
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