ArticleFrontiers in neuroenergetics2014
Induction of ischemic stroke in awake freely moving mice reveals that isoflurane anesthesia can mask the benefits of a neuroprotection therapy.
Article in Frontiers in neuroenergetics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 59 citations in OpenAlex.
- Knockdown of endothelial Serpine1 improves stroke recovery by attenuating peri-infarct blood flow and blood-brain barrier disruption.Cell death & disease · 2026Article
- Inducing and monitoring photothrombotic stroke in anesthetic neuroprotection-free mice using functional photoacoustic microscopy.Neurophotonics · 2026Article
- Hemodynamic and Electrophysiological Progression of the Rose Bengal Photothrombotic Stroke Model in Mice.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2026Article
- A minimally invasive thrombotic model to study stroke in awake mice.Nature communications · 2025Article
- Contralesional hippocampal spreading depolarization promotes functional recovery after stroke.Nature communications · 2025Article
- Biological and Procedural Predictors of Outcome in the Stroke Preclinical Assessment Network (SPAN) Trial.Circulation research · 2024Article
- Increased sensitivity in detection of deficits following two commonly used animal models of stroke.Behavioural brain research · 2024Article
- Brain-wide continuous functional ultrasound imaging for real-time monitoring of hemodynamics during ischemic stroke.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2024Article
- Article
- Perioperative stroke: A perspective on challenges and opportunities for experimental treatment and diagnostic strategies.CNS neuroscience & therapeutics · 2022Review
- Depth-targeted intracortical microstroke by two-photon photothrombosis in rodent brain.Neurophotonics · 2022Article
- Optical measurement of microvascular oxygenation and blood flow responses in awake mouse cortex during functional activation.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2022Article
- Dynamics of isoflurane-induced vasodilation and blood flow of cerebral vasculature revealed by multi-exposure speckle imaging.Journal of neuroscience methods · 2022Article
- Longitudinal functional imaging of VIP interneurons reveals sup-population specific effects of stroke that are rescued with chemogenetic therapy.Nature communications · 2021Article
- Frequency selective neuronal modulation triggers spreading depolarizations in the rat endothelin-1 model of stroke.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2021Article
- Preclinical animal studies in ischemic stroke: Challenges and some solutions.Animal models and experimental medicine · 2021Review
- Temporal Progression of Excitotoxic Calcium Following Distal Middle Cerebral Artery Occlusion in Freely Moving Mice.Frontiers in cellular neuroscience · 2020Article
- Awake chronic mouse model of targeted pial vessel occlusion via photothrombosis.Neurophotonics · 2020Article
- Control of occlusion of middle cerebral artery in perinatal and neonatal mice with magnetic force.Molecular brain · 2018Article
- Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Anesthetics such as isoflurane are commonly used to sedate experimental animals during the induction of stroke. Since these agents are known to modulate synaptic excitability, inflammation and blood flow, they could hinder the development and discovery of new neuroprotection therapies. To address this issue, we developed a protocol for inducing photothrombotic occlusion of cerebral vessels in fully conscious mice and tested two potential neuroprotectant drugs (a GluN2B or α4β2 nicotinic receptor antagonist). Our data show in vehicle treated mice that just 20 min of exposure to isoflurane during stroke induction can significantly reduce ischemic cortical damage relative to mice that were awake during stroke. When comparing potential stroke therapies, none provided any level of neuroprotection if the stroke was induced with anesthesia. However, if mice were fully conscious during stroke, the α4β2 nicotinic receptor antagonist reduced ischemic damage by 23% relative to vehicle treated controls, whereas the GluN2B antagonist had no significant effect. These results suggest that isoflurane anesthesia can occlude the benefits of certain stroke treatments and warrant caution when using anesthetics for pre-clinical testing of neuroprotective agents.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.