ArticleContrast media & molecular imaging
Minimization of self-quenching fluorescence on dyes conjugated to biomolecules with multiple labeling sites via asymmetrically charged NIR fluorophores.
Article in Contrast media & molecular imaging. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 80 citations in OpenAlex.
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- Human stem cells loaded with visible and near-infrared fluorescent core-shell polymeric nanoparticles and revealing tropism toward inflammatory stimuli: in vitro evaluation.Stem cell research & therapy · 2025Article
- The Medical Basis for the Photoluminescence of Indocyanine Green.Molecules (Basel, Switzerland) · 2025Review
- Evidence for a transfer-to-trap mechanism of fluorophore concentration quenching in lipid bilayers.Biophysical journal · 2024Article
- Preclinical evaluation of EpCAM-binding designed ankyrin repeat proteins (DARPins) as targeting moieties for bimodal near-infrared fluorescence and photoacoustic imaging of cancer.European journal of nuclear medicine and molecular imaging · 2024Article
- The current status and future prospects for molecular imaging-guided precision surgery.Cancer imaging : the official publication of the International Cancer Imaging Society · 2022Review
- In Situ Fluorescent Illumination of Microplastics in Water Utilizing a Combination of Dye/Surfactant and Quenching Techniques.Polymers · 2022Article
- Survey of X-ray induced Cherenkov excited fluorophores with potential for human use.Journal of radiation research · 2021Article
- Affinity-Based Methods for Site-Specific Conjugation of Antibodies.Bioconjugate chemistry · 2021Review
- Molecular probes for selective detection of cysteine cathepsins.Organic & biomolecular chemistry · 2021Review
- Heteroacene-Based Amphiphile as a Molecular Scaffold for Bioimaging Probes.Frontiers in chemistry · 2021Article
- Direct evaluation of self-quenching behavior of fluorophores at high concentrations using an evanescent field.PloS one · 2021Article
- Amphiphilic "Like-a-Brush" Oligonucleotide Conjugates with Three Dodecyl Chains: Self-Assembly Features of Novel Scaffold Compounds for Nucleic Acids Delivery.Nanomaterials (Basel, Switzerland) · 2020Article
- Hybrid Multimodal Imaging Synthons for Chemoselective and Efficient Biomolecule Modification with Chelator and Near-Infrared Fluorescent Cyanine Dye.Pharmaceuticals (Basel, Switzerland) · 2020Article
- Articular Cartilage- and Synoviocyte-Binding Poly(ethylene glycol) Nanocomposite Microgels as Intra-Articular Drug Delivery Vehicles for the Treatment of Osteoarthritis.ACS biomaterials science & engineering · 2020Article
- Analysis of Flavonoid Compounds by Terahertz Spectroscopy Combined with Chemometrics.ACS omega · 2020Article
- DNA Photocleavage in the Near-Infrared Wavelength Range by 2-Quinolinium Dicarbocyanine Dyes.Molecules (Basel, Switzerland) · 2020Article
- Design and Applications of a Fluorescent Labeling Technique for Lipid and Surfactant Preformed Vesicles.ACS omega · 2019Article
- Near-Infrared-Fluorescent Erythrocyte-Mimicking Particles: Physical and Optical Characteristics.IEEE transactions on bio-medical engineering · 2019Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Self-aggregation of dyes even at low concentrations poses a considerable challenge in preparing sufficiently bright molecular probes for in vivo imaging, particularly in the conjugation of near infrared cyanine dyes to polypeptides with multiple labeling sites. Such self-aggregation leads to a significant energy transfer between the dyes, resulting in severe quenching and low brightness of the targeted probe. To address this problem, we designed a novel type of dye with an asymmetrical distribution of charge. Asymmetrical distribution prevents the chromophores from π-stacking thus minimizing the energy transfer and fluorescence quenching. The conjugation of the dye to polypeptides showed only a small presence of an H-aggregate band in the absorption spectra and, hence, a relatively high quantum efficiency.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.