ArticleDevelopment (Cambridge, England)2014
Highly efficient targeted mutagenesis in axolotl using Cas9 RNA-guided nuclease.
Article in Development (Cambridge, England), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
55 citing papers in PubMed.
- Optimized toolkit for the manipulation of immortalized axolotl fibroblasts.Methods (San Diego, Calif.) · 2025Article
- Article
- Limb blastema formation: How much do we know at a genetic and epigenetic level?The Journal of biological chemistry · 2023Review
- Now that We Got There, What Next?Methods in molecular biology (Clifton, N.J.) · 2023Article
- Salamanders as Key Models for Development and Regeneration Research.Methods in molecular biology (Clifton, N.J.) · 2023Review
- A Practical Guide for CRISPR-Cas9-Induced Mutations in Axolotls.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Hybridization Chain Reaction Fluorescence In Situ Hybridization (HCR-FISH) in Ambystoma mexicanum Tissue.Methods in molecular biology (Clifton, N.J.) · 2023Article
- The use of transgenics in the laboratory axolotl.Developmental dynamics : an official publication of the American Association of Anatomists · 2022Review
- The amazing and anomalous axolotls as scientific models.Developmental dynamics : an official publication of the American Association of Anatomists · 2022Article
- Gene and transgenics nomenclature for the laboratory axolotl-Ambystoma mexicanum.Developmental dynamics : an official publication of the American Association of Anatomists · 2022Article
- The Axolotl's journey to the modern molecular era.Current topics in developmental biology · 2022Article
- Towards comparative analyses of salamander limb regeneration.Journal of experimental zoology. Part B, Molecular and developmental evolution · 2021Review
- Review
- Salamanders: The molecular basis of tissue regeneration and its relevance to human disease.Current topics in developmental biology · 2021Review
- CRISPR/Cas System and Factors Affecting Its Precision and Efficiency.Frontiers in cell and developmental biology · 2021Review
- What the salamander eye has been telling the vision scientist's brain.Seminars in cell & developmental biology · 2020Review
- Article
- Generating Stable Knockout Zebrafish Lines by Deleting Large Chromosomal Fragments Using Multiple gRNAs.G3 (Bethesda, Md.) · 2020Article
- Article
- Advancements to the Axolotl Model for Regeneration and Aging.Gerontology · 2020Review
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Among tetrapods, only urodele salamanders, such as the axolotl Ambystoma mexicanum, can completely regenerate limbs as adults. The mystery of why salamanders, but not other animals, possess this ability has for generations captivated scientists seeking to induce this phenomenon in other vertebrates. Although many recent advances in molecular biology have allowed limb regeneration and tissue repair in the axolotl to be investigated in increasing detail, the molecular toolkit for the study of this process has been limited. Here, we report that the CRISPR-Cas9 RNA-guided nuclease system can efficiently create mutations at targeted sites within the axolotl genome. We identify individual animals treated with RNA-guided nucleases that have mutation frequencies close to 100% at targeted sites. We employ this technique to completely functionally ablate EGFP expression in transgenic animals and recapitulate developmental phenotypes produced by loss of the conserved gene brachyury. Thus, this advance allows a reverse genetic approach in the axolotl and will undoubtedly provide invaluable insight into the mechanisms of salamanders' unique regenerative ability.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.