ArticlePLoS pathogens2014
The transcriptional activator LdtR from 'Candidatus Liberibacter asiaticus' mediates osmotic stress tolerance.
Article in PLoS pathogens, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 59 citations in OpenAlex.
- Control efficiency and Huanglongbing resistance-related clues mediated by novelFrontiers in microbiology · 2026Article
- Recent progress in the understanding of Citrus Huanglongbing: from the perspective of pathogen and citrus host.Molecular breeding : new strategies in plant improvement · 2024Article
- Review
- Investigation of the anti-Huanglongbing effects using antimicrobial lipopeptide and phytohormone complex powder prepared fromFrontiers in microbiology · 2024Article
- A protease and a lipoprotein jointly modulate the conserved ExoR-ExoS-ChvI signaling pathway critical in Sinorhizobium meliloti for symbiosis with legume hosts.PLoS genetics · 2023Article
- Pathogenicity and Transcriptomic Analyses of Two "Microbiology spectrum · 2023Article
- Article
- Biological Features and In Planta Transcriptomic Analyses of aInternational journal of molecular sciences · 2022Article
- Osmotic stress induces long-term biofilm survival in Liberibacter crescens.BMC microbiology · 2022Article
- A serralysin-like protein ofFrontiers in microbiology · 2022Article
- An Overview of the Mechanisms Against "Frontiers in microbiology · 2022Review
- Zinkicide Is a ZnO-Based Nanoformulation with Bactericidal Activity against Liberibacter crescens in Batch Cultures and in Microfluidic Chambers Simulating Plant Vascular Systems.Applied and environmental microbiology · 2020Article
- Method Optimization: Analysis of Benzbromarone and Tolfenamic Acid in Citrus Tissues and Soil Using Liquid Chromatography Coupled With Triple-Quadrupole Mass Spectrometry.Frontiers in plant science · 2020Article
- A high-throughput system to identify inhibitors ofProceedings of the National Academy of Sciences of the United States of America · 2019Article
- The Ferredoxin-Like Protein FerR Regulates PrbP Activity inApplied and environmental microbiology · 2019Article
- Nuclear Import of a Secreted "Frontiers in microbiology · 2019Article
- Purification and partial characterization of LdtP, a cell envelope modifying enzyme in Liberibacter asiaticus.BMC microbiology · 2018Article
- Functional Annotation of Bacterial Signal Transduction Systems: Progress and Challenges.International journal of molecular sciences · 2018Review
- Novel Genes and Regulators That Influence Production of Cell Surface Exopolysaccharides in Sinorhizobium meliloti.Journal of bacteriology · 2018Article
- Development of Chemically Defined Media Reveals Citrate as Preferred Carbon Source forFrontiers in microbiology · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The causal agent of Huanglongbing disease, 'Candidatus Liberibacter asiaticus', is a non-culturable, gram negative, phloem-limited α-proteobacterium. Current methods to control the spread of this disease are still limited to the removal and destruction of infected trees. In this study, we identified and characterized a regulon from 'Ca. L. asiaticus' involved in cell wall remodeling, that contains a member of the MarR family of transcriptional regulators (ldtR), and a predicted L,D-transpeptidase (ldtP). In Sinorhizobium meliloti, mutation of ldtR resulted in morphological changes (shortened rod-type phenotype) and reduced tolerance to osmotic stress. A biochemical approach was taken to identify small molecules that modulate LdtR activity. The LdtR ligands identified by thermal shift assays were validated using DNA binding methods. The biological impact of LdtR inactivation by the small molecules was then examined in Sinorhizobium meliloti and Liberibacter crescens, where a shortened-rod phenotype was induced by growth in presence of the ligands. A new method was also developed to examine the effects of small molecules on the viability of 'Ca. Liberibacter asiaticus', using shoots from HLB-infected orange trees. Decreased expression of ldtRLas and ldtPLas was observed in samples taken from HLB-infected shoots after 6 h of incubation with the LdtR ligands. These results provide strong proof of concept for the use of small molecules that target LdtR, as a potential treatment option for Huanglongbing disease.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.