ArticleProtoplasma2014
Morpho-histology and genotype dependence of in vitro morphogenesis in mature embryo cultures of wheat.
Article in Protoplasma, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 68 citations in OpenAlex.
- Molecular Mechanisms of Programmed Cell Death During Somatic Embryo Detachment from Callus in Indirect Somatic Embryogenesis.International journal of molecular sciences · 2026Review
- Molecular regulators of regeneration and strategies for overcoming genotype-dependent recalcitrance in wheat (Triticum aestivum).Communications biology · 2026Review
- Molecular Regulators of In Vitro Regeneration in Wheat: Roles of Morphogenic Factors in Transformation, Genome Editing, and Breeding.International journal of molecular sciences · 2026Review
- Somatic Embryogenesis and Genetic Transformation ofPlants (Basel, Switzerland) · 2025Article
- Form and Function: The Factors That Influence the Efficacy of Nanomaterials for Gene Transfer to Plants.Molecules (Basel, Switzerland) · 2025Review
- Modulation of Plant MicroRNA Expression: Its Potential Usability in Wheat (Current genomics · 2023Review
- Investigation of the Effect of the Auxin Antagonist PEO-IAA on Cannabinoid Gene Expression and Content inPlants (Basel, Switzerland) · 2023Article
- Efficient protocol ofFrontiers in plant science · 2023Article
- Cryo-derived plants through embryogenesis showed same levels of vinblastine and vincristine (anticancer) in Catharanthus roseus and had normal genome size.Scientific reports · 2022Article
- Applications of In Vitro Tissue Culture Technologies in Breeding and Genetic Improvement of Wheat.Plants (Basel, Switzerland) · 2022Review
- The Promising Nanovectors for Gene Delivery in Plant Genome Engineering.International journal of molecular sciences · 2022Review
- Internal and External Regulatory Elements Controlling Somatic Embryogenesis in Catharanthus: A Model Medicinal Plant.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Highly efficient and genotype-independent barley gene editing based on anther culture.Plant communications · 2021Article
- A competence of embryo-derived tissues of tetraploid cultivated wheat species Triticum dicoccum and Triticum timopheevii for efficient and stable transgenesis mediated by particle inflow gun.BMC plant biology · 2020Article
- Induction of Callogenesis, Organogenesis, and Embryogenesis in Non-Meristematic Explants of Bleeding Heart and Evaluation of Chemical Diversity of Key Metabolites from Callus.International journal of molecular sciences · 2020Article
- Genes, proteins and other networks regulating somatic embryogenesis in plants.Journal, genetic engineering & biotechnology · 2020Review
- Cellular, Molecular, and Physiological Aspects of In Vitro Plant Regeneration.Plants (Basel, Switzerland) · 2020Review
- Genome size analysis of field grown and somatic embryo regenerated plants in Allium sativum L.Journal of applied genetics · 2020Article
- De novo assembly and comparative analysis of the transcriptome of embryogenic callus formation in bread wheat (Triticum aestivum L.).BMC plant biology · 2017Article
- Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cellular totipotency is one of the basic principles of plant biotechnology. Currently, the success of the procedure used to produce transgenic plants is directly proportional to the successful insertion of foreign DNA into the genome of suitable target tissue/cells that are able to regenerate plants. The mature embryo (ME) is increasingly recognized as a valuable explant for developing regenerable cell lines in wheat biotechnology. We have previously developed a regeneration procedure based on fragmented ME in vitro culture. Before we can use this regeneration system as a model for molecular studies of the morphogenic pathway induced in vitro and investigate the functional links between regenerative capacity and transformation receptiveness, some questions need to be answered. Plant regeneration from cultured tissues is genetically controlled. Factors such as age/degree of differentiation and physiological conditions affect the response of explants to culture conditions. Plant regeneration in culture can be achieved through embryogenesis or organogenesis. In this paper, the suitability of ME tissues for tissue culture and the chronological series of morphological data observed at the macroscopic level are documented. Genetic variability at each step of the regeneration process was evaluated through a varietal comparison of several elite wheat cultivars. A detailed histological analysis of the chronological sequence of morphological events during ontogeny was conducted. Compared with cultures of immature zygotic embryos, we found that the embryogenic pathway occurs slightly earlier and is of a different origin in our model. Cytological, physiological, and some biochemical aspects of somatic embryo formation in wheat ME culture are discussed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.