ArticlePLoS genetics2014
Mutations in conserved residues of the C. elegans microRNA Argonaute ALG-1 identify separable functions in ALG-1 miRISC loading and target repression.
Article in PLoS genetics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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30 citing papers in PubMed, 46 citations in OpenAlex.
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- Dipeptidyl peptidase DPF-3 is a gatekeeper of microRNA Argonaute compensation in animals.Nature communications · 2025Article
- Catalytic residues of microRNA Argonautes play a modest role in microRNA star strand destabilization in C. elegans.Nucleic acids research · 2024Article
- An Intricate Network Involving the Argonaute ALG-1 Modulates Organismal Resistance to Oxidative Stress.Nature communications · 2024Article
- An RNAi screen for conserved kinases that enhance microRNA activity after dauer in Caenorhabditis elegans.G3 (Bethesda, Md.) · 2024Article
- Modeling neurodevelopmental disorder-associated humanProceedings of the National Academy of Sciences of the United States of America · 2024Article
- The catalytic activity of microRNA Argonautes plays a modest role in microRNA star strand destabilization inbioRxiv : the preprint server for biology · 2024Article
- A specific type of Argonaute phosphorylation regulates binding to microRNAs during C. elegans development.Cell reports · 2022Article
- Functional identification of microRNA-centered complexes in C. elegans.Scientific reports · 2022Article
- Global profiling and annotation of templated isomiRs dynamics acrossRNA biology · 2022Article
- PQN-59 antagonizes microRNA-mediated repression during post-embryonic temporal patterning and modulates translation and stress granule formation in C. elegans.PLoS genetics · 2021Article
- Recovery from heat shock requires the microRNA pathway in Caenorhabditis elegans.PLoS genetics · 2021Article
- microRNA strand selection: Unwinding the rules.Wiley interdisciplinary reviews. RNA · 2021Review
- Cell-type-specific profiling of loaded miRNAs from Caenorhabditis elegans reveals spatial and temporal flexibility in Argonaute loading.Nature communications · 2021Article
- KH domain containing RNA-binding proteins coordinate with microRNAs to regulate Caenorhabditis elegans development.G3 (Bethesda, Md.) · 2021Article
- Protein Extract Preparation and Co-immunoprecipitation from Caenorhabditis elegans.Journal of visualized experiments : JoVE · 2020Article
- HRPK-1, a conserved KH-domain protein, modulates microRNA activity during Caenorhabditis elegans development.PLoS genetics · 2019Article
- Recent Molecular Genetic Explorations ofGenetics · 2018Article
- Opposing roles of microRNA Argonautes during Caenorhabditis elegans aging.PLoS genetics · 2018Article
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Authors and funding
5 authors at 4 institutions in 2 countries.
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Abstract
microRNAs function in diverse developmental and physiological processes by regulating target gene expression at the post-transcriptional level. ALG-1 is one of two Caenorhabditis elegans Argonautes (ALG-1 and ALG-2) that together are essential for microRNA biogenesis and function. Here, we report the identification of novel antimorphic (anti) alleles of ALG-1 as suppressors of lin-28(lf) precocious developmental phenotypes. The alg-1(anti) mutations broadly impair the function of many microRNAs and cause dosage-dependent phenotypes that are more severe than the complete loss of ALG-1. ALG-1(anti) mutant proteins are competent for promoting Dicer cleavage of microRNA precursors and for associating with and stabilizing microRNAs. However, our results suggest that ALG-1(anti) proteins may sequester microRNAs in immature and functionally deficient microRNA Induced Silencing Complexes (miRISCs), and hence compete with ALG-2 for access to functional microRNAs. Immunoprecipitation experiments show that ALG-1(anti) proteins display an increased association with Dicer and a decreased association with AIN-1/GW182. These findings suggest that alg-1(anti) mutations impair the ability of ALG-1 miRISC to execute a transition from Dicer-associated microRNA processing to AIN-1/GW182 associated effector function, and indicate an active role for ALG/Argonaute in mediating this transition.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.