ArticlePLoS neglected tropical diseases2014
Presence of extensive Wolbachia symbiont insertions discovered in the genome of its host Glossina morsitans morsitans.
Article in PLoS neglected tropical diseases, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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44 citing papers in PubMed, 87 citations in OpenAlex.
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- Establishment and partial characterisation of a new cell line derived from adult tissues of the tsetse fly Glossina morsitans morsitans.Parasites & vectors · 2024Article
- A Light in the Dark: Uncovering Wolbachia-Host Interactions Using Fluorescence Imaging.Methods in molecular biology (Clifton, N.J.) · 2024Article
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- Living in the endosymbiotic world of Wolbachia: A centennial review.Cell host & microbe · 2021Review
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- Bacterial Symbionts of Tsetse Flies: Relationships and Functional Interactions Between Tsetse Flies and Their Symbionts.Results and problems in cell differentiation · 2020Review
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- Detection and characterization of bacterial endosymbionts in Southeast Asian tephritid fruit fly populations.BMC microbiology · 2019Article
- Genome Assembly of the A-Group Wolbachia in Nasonia oneida Using Linked-Reads Technology.Genome biology and evolution · 2019Article
- Comparative genomic analysis of six Glossina genomes, vectors of African trypanosomes.Genome biology · 2019Article
- The first draft genomes of the ant Formica exsecta, and its Wolbachia endosymbiont reveal extensive gene transfer from endosymbiont to host.BMC genomics · 2019Article
- Nuclear and Wolbachia-based multimarker approach for the rapid and accurate identification of tsetse species.BMC microbiology · 2018Article
- Enhancing vector refractoriness to trypanosome infection: achievements, challenges and perspectives.BMC microbiology · 2018Article
- High-sensitivity detection of cryptic Wolbachia in the African tsetse fly (Glossina spp.).BMC microbiology · 2018Article
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Authors and funding
14 authors at 7 institutions in 6 countries.
Funding
Abstract
Tsetse flies (Glossina spp.) are the cyclical vectors of Trypanosoma spp., which are unicellular parasites responsible for multiple diseases, including nagana in livestock and sleeping sickness in humans in Africa. Glossina species, including Glossina morsitans morsitans (Gmm), for which the Whole Genome Sequence (WGS) is now available, have established symbiotic associations with three endosymbionts: Wigglesworthia glossinidia, Sodalis glossinidius and Wolbachia pipientis (Wolbachia). The presence of Wolbachia in both natural and laboratory populations of Glossina species, including the presence of horizontal gene transfer (HGT) events in a laboratory colony of Gmm, has already been shown. We herein report on the draft genome sequence of the cytoplasmic Wolbachia endosymbiont (cytWol) associated with Gmm. By in silico and molecular and cytogenetic analysis, we discovered and validated the presence of multiple insertions of Wolbachia (chrWol) in the host Gmm genome. We identified at least two large insertions of chrWol, 527,507 and 484,123 bp in size, from Gmm WGS data. Southern hybridizations confirmed the presence of Wolbachia insertions in Gmm genome, and FISH revealed multiple insertions located on the two sex chromosomes (X and Y), as well as on the supernumerary B-chromosomes. We compare the chrWol insertions to the cytWol draft genome in an attempt to clarify the evolutionary history of the HGT events. We discuss our findings in light of the evolution of Wolbachia infections in the tsetse fly and their potential impacts on the control of tsetse populations and trypanosomiasis.
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