ArticlePloS one2014
Dietary flavanols modulate the transcription of genes associated with cardiovascular pathology without changes in their DNA methylation state.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 2 of them syntheses that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Pooled it
- Dietary Flavonols Intake and Risk of Esophageal and Gastric Cancer: A Meta-Analysis of Epidemiological Studies.Nutrients · 2016Pooled it
- Gene expression changes by high-polyphenols cocoa powder intake: a randomized crossover clinical study.European journal of nutrition · 2019Trial
- Article
- Examining nutrition strategies to influence DNA methylation and epigenetic clocks: a systematic review of clinical trials.Frontiers in aging · 2024Review
- Eat4Genes: a bioinformatic rational gene targeting app and prototype model for improving human health.Frontiers in nutrition · 2023Article
- Flavanol Consumption in Healthy Men Preserves Integrity of Immunological-Endothelial Barrier Cell Functions: Nutri(epi)genomic Analysis.Molecular nutrition & food research · 2022Article
- Review
- The link among microbiota, epigenetics, and disease development.Environmental science and pollution research international · 2021Review
- Echinacea purpurea (L.) Moench treatment of monocytes promotes tonic interferon signaling, increased innate immunity gene expression and DNA repeat hypermethylated silencing of endogenous retroviral sequences.BMC complementary medicine and therapies · 2021Article
- Cloning and expression analysis of GATA1 gene in Carassius auratus red var.BMC genomic data · 2021Article
- Differential Induction Pattern Towards Classically Activated Macrophages in Response to an Immunomodulatory Extract fromJournal of fungi (Basel, Switzerland) · 2021Article
- Detection of suspicious interactions of spiking covariates in methylation data.BMC bioinformatics · 2020Article
- DNA methylation, colon cancer and Mediterranean diet: results from the EPIC-Italy cohort.Epigenetics · 2019Article
- Article
- Pro-Apoptotic Effect of Grape Seed Extract on MCF-7 Involves Transient Increase of Gap Junction Intercellular Communication and Cx43 Up-Regulation: A Mechanism of Chemoprevention.International journal of molecular sciences · 2019Article
- A systems biology network analysis of nutri(epi)genomic changes in endothelial cells exposed to epicatechin metabolites.Scientific reports · 2018Article
- Article
- DNA methylation as a marker of response in rheumatoid arthritis.Pharmacogenomics · 2017Review
- An epigenome-wide association study of inflammatory response to fenofibrate in the Genetics of Lipid Lowering Drugs and Diet Network.Pharmacogenomics · 2017Article
Corrections and comments
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Authors and funding
17 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIn a recent intervention study, the daily supplementation with 200 mg monomeric and oligomeric flavanols (MOF) from grape seeds for 8 weeks revealed a vascular health benefit in male smokers. The objective of the present study was to determine the impact of MOF consumption on the gene expression profile of leukocytes and to assess changes in DNA methylation. METHODOLOGY/PRINCIPAL
findingsGene expression profiles were determined using whole genome microarrays (Agilent) and DNA methylation was assessed using HumanMethylation450 BeadChips (Illumina). MOF significantly modulated the expression of 864 genes. The majority of the affected genes are involved in chemotaxis, cell adhesion, cell infiltration or cytoskeleton organisation, suggesting lower immune cell adhesion to endothelial cells. This was corroborated by in vitro experiments showing that MOF exposure of monocytes attenuates their adhesion to TNF-α-stimulated endothelial cells. Nuclear factor kappa B (NF-κB) reporter gene assays confirmed that MOF decrease the activity of NF-κB. Strong inter-individual variability in the leukocytes' DNA methylation was observed. As a consequence, on group level, changes due to MOF supplementation could not be found.
conclusionOur study revealed that an 8 week daily supplementation with 200 mg MOF modulates the expression of genes associated with cardiovascular disease pathways without major changes of their DNA methylation state. However, strong inter-individual variation in leukocyte DNA methylation may obscure the subtle epigenetic response to dietary flavanols. Despite the lack of significant changes in DNA methylation, the modulation of gene expression appears to contribute to the observed vascular health effect of MOF in humans.
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